Bioactivity guided fractionation for anti-tumour and toxicity evaluation of selected extracts of Hydnophytum formicarium Jack Sanola


Citation

Azimahtol Hawariah L. P., . and Ng Y., . (2000) Bioactivity guided fractionation for anti-tumour and toxicity evaluation of selected extracts of Hydnophytum formicarium Jack Sanola. [Proceedings Paper]

Abstract

The whole plant of Hydnophytum formicarium Jack Rubiaceae locally known as Sanola was extracted using both polar and non-polar solvents. The crude extracts were tested in vitro for anti-proliferative activity. Results showed that methanolic n-butanolic and aqueous crude extracts showed anti-proliferative effect towards Caov-3 ovarian cancer and KB-IV epidermoid carcinoma weak against DU-154 prostate cancer and MCF-7 breast cancer and interestingly no effect on normal cells MDBK and Vero cells. For the mode of action study the methanolic extract was tested for its effect on apoptosis on human ovarian cancer cell line Caov-3 using TUNEL assay. The results indicated that cell death was achieved through the mechanism of apoptosis.


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Abstract

The whole plant of Hydnophytum formicarium Jack Rubiaceae locally known as Sanola was extracted using both polar and non-polar solvents. The crude extracts were tested in vitro for anti-proliferative activity. Results showed that methanolic n-butanolic and aqueous crude extracts showed anti-proliferative effect towards Caov-3 ovarian cancer and KB-IV epidermoid carcinoma weak against DU-154 prostate cancer and MCF-7 breast cancer and interestingly no effect on normal cells MDBK and Vero cells. For the mode of action study the methanolic extract was tested for its effect on apoptosis on human ovarian cancer cell line Caov-3 using TUNEL assay. The results indicated that cell death was achieved through the mechanism of apoptosis.

Additional Metadata

[error in script]
Item Type: Proceedings Paper
Additional Information: Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal QK99 A1S471 2000 Call Number.
AGROVOC Term: TISSUE PROLIFERATION
AGROVOC Term: APOPTOSIS
AGROVOC Term: RUBIACEAE
AGROVOC Term: IN VITRO
AGROVOC Term: MALAYSIA
Geographical Term: MALAYSIA
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 05:14
URI: http://webagris.upm.edu.my/id/eprint/11320

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