Bioautographic profile as standard reference for quantitative analysis of the efficacy of herbs as antiseptic and antioxidant

Nurhaya M T., . and Aina Fairuz A. L., . and Norazian M H., . and Hasdayanti S., . and Sha`erah M Y., . (2009) Bioautographic profile as standard reference for quantitative analysis of the efficacy of herbs as antiseptic and antioxidant. [Proceedings Paper]

This study is designed to develop a standard reference for qualitative analysis on the efficacy of herbs as antiseptic and antioxidant with the aim to validate the authenticity of the herbal materials and quality control of their pharmaceutical herbal preparations. A method comprising a combination of bioautographic assay and thin layer chromatography TLC analysis was employd to detect the antioxidant and antiseptic biomarkers as well as to obtain the TLC profile. The herbal extracts were subjected to TLC analysis on silica gel plates and each separated compound was visualized by various detection methods.Various extract from three selected herbs namely piper betle L. leaves.Ficus deltoidea Jack. leaves and Ruta angustifolia L Pers. aerial parts were studied. Antiseptic biomakers were were detected by using bioautographic agar overlay assay against selected standard bacterial and fungal strains namely Staphylococcus aureus. Escherichia colli Candida albicans Microsporum canis and Triscophyton rubrum. The antioxidant biomarkers were determined using TLC and 2.2-depheny1-2-picrylhydrazy1 DPPH staining assay. The complete biographic profile which included the antioxidant and antiseptic biomarkers with their TLC characteristic were used as standard reference for qualitative analysis on the efficacy of the herbs and and their efficacy and their herbal preparation. This method would also be applicable to other herbs for efficacy analysis on both of the medicinal properties tested here.

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Abstract

This study is designed to develop a standard reference for qualitative analysis on the efficacy of herbs as antiseptic and antioxidant with the aim to validate the authenticity of the herbal materials and quality control of their pharmaceutical herbal preparations. A method comprising a combination of bioautographic assay and thin layer chromatography TLC analysis was employd to detect the antioxidant and antiseptic biomarkers as well as to obtain the TLC profile. The herbal extracts were subjected to TLC analysis on silica gel plates and each separated compound was visualized by various detection methods.Various extract from three selected herbs namely piper betle L. leaves.Ficus deltoidea Jack. leaves and Ruta angustifolia L Pers. aerial parts were studied. Antiseptic biomakers were were detected by using bioautographic agar overlay assay against selected standard bacterial and fungal strains namely Staphylococcus aureus. Escherichia colli Candida albicans Microsporum canis and Triscophyton rubrum. The antioxidant biomarkers were determined using TLC and 2.2-depheny1-2-picrylhydrazy1 DPPH staining assay. The complete biographic profile which included the antioxidant and antiseptic biomarkers with their TLC characteristic were used as standard reference for qualitative analysis on the efficacy of the herbs and and their efficacy and their herbal preparation. This method would also be applicable to other herbs for efficacy analysis on both of the medicinal properties tested here.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal QK99 M3S471 2008 Call Number
AGROVOC Term:	QUALITATIVE ANALYSIS
AGROVOC Term:	HERBACEOUS PLANTS
AGROVOC Term:	DISINFECTANTS
AGROVOC Term:	ANTIOXIDANTS
AGROVOC Term:	MALAYSIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/11348

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