Antioxidant properties of leaf crude extracts of Piper nigrum L. Piperaceae

Radzali M., . and Nur Dalila Hani M Z., . (2009) Antioxidant properties of leaf crude extracts of Piper nigrum L. Piperaceae. [Proceedings Paper]

Piper nigrum L. black pepper is a perennial woody climber that possesses medicinal value. The fresh leaves of P. nigrum were collected from Taman Pertanian Universiti TPU Universiti Putra Malaysia Serdang Selangor. The leaves were extracted with four different solvents ie. methanol ethanol hexane and distilled water to obtain antioxidant compounds. Polar solvents such as methanol ethanol and boiling water exhibited higher extraction yields compared to non-polar solvent hexane. Among the three types of solvents the methanolic extracts exhibited the highest yield 16.91 2.27. Total phenolic content and total flavonoid content determination were carried out on the extracts to determine the total antioxidant compounds present in P. nigrum leaves. Methanolic extracts exhibited the highest total phenolic content 1.96 0.05 mg GAE/g dw of tissue and total flavonoid content 1.51 0.0lmg QE/g dw of tissue. The total flavonoid content of all polar solvents were almost the same value as the methanol extract. Antioxidant activity of P. nigrum leaves was determined by the free radical scavenging ability of 1 1-diphenyl-2-picrylhydrazyl DPPH as well as lipid peroxidation inhabition using thiobarbituric acid TBA. The methanolic extracts showed the highest antioxidant activity in both DPPH and TBA assays 74.22 4.50and 55.48 5.05 respectively. The leaf extract of P. nigrum contain natural compounds that exhibited antioxidant activity. P. nigrum extracts showed higher antioxidant potential in free radical scavenging compared to inhabition of lipid peroxidation.

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Abstract

Piper nigrum L. black pepper is a perennial woody climber that possesses medicinal value. The fresh leaves of P. nigrum were collected from Taman Pertanian Universiti TPU Universiti Putra Malaysia Serdang Selangor. The leaves were extracted with four different solvents ie. methanol ethanol hexane and distilled water to obtain antioxidant compounds. Polar solvents such as methanol ethanol and boiling water exhibited higher extraction yields compared to non-polar solvent hexane. Among the three types of solvents the methanolic extracts exhibited the highest yield 16.91 2.27. Total phenolic content and total flavonoid content determination were carried out on the extracts to determine the total antioxidant compounds present in P. nigrum leaves. Methanolic extracts exhibited the highest total phenolic content 1.96 0.05 mg GAE/g dw of tissue and total flavonoid content 1.51 0.0lmg QE/g dw of tissue. The total flavonoid content of all polar solvents were almost the same value as the methanol extract. Antioxidant activity of P. nigrum leaves was determined by the free radical scavenging ability of 1 1-diphenyl-2-picrylhydrazyl DPPH as well as lipid peroxidation inhabition using thiobarbituric acid TBA. The methanolic extracts showed the highest antioxidant activity in both DPPH and TBA assays 74.22 4.50and 55.48 5.05 respectively. The leaf extract of P. nigrum contain natural compounds that exhibited antioxidant activity. P. nigrum extracts showed higher antioxidant potential in free radical scavenging compared to inhabition of lipid peroxidation.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal QK99 M3S471 2008 Call Number
AGROVOC Term:	Antioxidants
AGROVOC Term:	Piper nigrum
AGROVOC Term:	Leaves
AGROVOC Term:	Extracts
AGROVOC Term:	Piperaceae
AGROVOC Term:	Oxidation
AGROVOC Term:	Lipid content
AGROVOC Term:	Lipids
AGROVOC Term:	Malaysia
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/11373

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