Citation
Salbiah M., . and Fauziah A., . and Ling S K., . (2009) Chemical fingerprinting of Piper muricatum Blume by fourier transform infrared spectroscopy with two dimensional correlation IR spectroscopy and high performance liquid chromatography. [Proceedings Paper]
Abstract
Piper muricafum Blume of the family Piperaceae locally known as Sireh hutan is a perennial climber rarely a shrub and is reported as one of the wild Piper species found in Malaysian forest. Preliminary biological studies have demonstrated that methanol extracts of leaves and stem of this plant exhibited therapeutic properties such as anti-microbial anti-diabetic. anti-inflammatory as well as anti-oxidant. Fourier transform infrared spectroscopy FT-IR associated with second derivative infrared spectroscopy and two dimensional correlation infrared spectroscopy 2D-IR were applied to study the macroscopic fingerprint of the raw materials of P.muricatum. The macroscopic fingerprint of FT-IR and 2D-IR spectra have indicated many characteristic and holistic variation rules of chemical constituents in the stem and leaves and these were used to identify the main chemical constituents present in the plant material This analytical method is highly rapid effective visual and accurate for medicinal plant research. This study also attempts to develop HPLC fingerprint of the stem and leaves of P. muricatum. Two distinct peaks with UV spectra detected at wavelength 204 nm and 284 nm were found in the methanol extract of the leaves whilst one distinct peak with UV spectrum at wavelength 204 nm and 284 nm was found in the stem.These compounds would be further characterized and could be used as markers for standardisation of this species.
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Abstract
Piper muricafum Blume of the family Piperaceae locally known as Sireh hutan is a perennial climber rarely a shrub and is reported as one of the wild Piper species found in Malaysian forest. Preliminary biological studies have demonstrated that methanol extracts of leaves and stem of this plant exhibited therapeutic properties such as anti-microbial anti-diabetic. anti-inflammatory as well as anti-oxidant. Fourier transform infrared spectroscopy FT-IR associated with second derivative infrared spectroscopy and two dimensional correlation infrared spectroscopy 2D-IR were applied to study the macroscopic fingerprint of the raw materials of P.muricatum. The macroscopic fingerprint of FT-IR and 2D-IR spectra have indicated many characteristic and holistic variation rules of chemical constituents in the stem and leaves and these were used to identify the main chemical constituents present in the plant material This analytical method is highly rapid effective visual and accurate for medicinal plant research. This study also attempts to develop HPLC fingerprint of the stem and leaves of P. muricatum. Two distinct peaks with UV spectra detected at wavelength 204 nm and 284 nm were found in the methanol extract of the leaves whilst one distinct peak with UV spectrum at wavelength 204 nm and 284 nm was found in the stem.These compounds would be further characterized and could be used as markers for standardisation of this species.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal QK99 M3S471 2008 Call Number |
| AGROVOC Term: | PIPER |
| AGROVOC Term: | INFRARED SPECTROPHOTOMETRY |
| AGROVOC Term: | CHROMATOGRAPHY |
| AGROVOC Term: | PIPERACEAE |
| AGROVOC Term: | MALAYSIA |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:14 |
| URI: | http://webagris.upm.edu.my/id/eprint/11378 |
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