Citation
Ihsan Safwan K., . and Mazura M P., . and Fadzureena J., . and Khoo M G H., . (2009) Evaluation of anti-inflammatory and cytotoxic activities of stem and leaf extracts of a plant from the family Thymelaeaceae. [Proceedings Paper]
Abstract
DT08/540 stem and DT08/541 leaf are the crude extracts of a plant from the family Thymelaeaceae. This plant is traditionally used in aroma-therapy. The present study sought to evaluate the efficacy and safety of these extracts. Methanol extracts of this plant were evaluated for anti-inflammatory activity based on hyaluronidase lipoxygenase and xanthine oxidase inhibitory assays. The leaf extract DT08/541 showed strong inhibition on hyaluronidase lipoxygenase and xanthine oxidase with inhibition of 96.42 4.08 81.34 0.01and 64. 13 0.01 respectively. However the stem extract DT08/541 exhibited low activity in all assays tested. Cytotoxic effect of the extracts was investigated in vitro using Sulforhodamine B SRB assay on normal kidney Vero and normal liver WRL-68 cell lines. The results showed the median inhibitory concentration IC50 of stem and leaf extracts in both cell lines ranged from 60 ug/ml to 375 ug/ml. Since both extracts showed a IC50 value above 50 ug/ml it could be considered as non-cytotoxic. This study showed that the leaf extracts of this plant could be further explored for potential usage as remedies for inflammation treatment.
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Abstract
DT08/540 stem and DT08/541 leaf are the crude extracts of a plant from the family Thymelaeaceae. This plant is traditionally used in aroma-therapy. The present study sought to evaluate the efficacy and safety of these extracts. Methanol extracts of this plant were evaluated for anti-inflammatory activity based on hyaluronidase lipoxygenase and xanthine oxidase inhibitory assays. The leaf extract DT08/541 showed strong inhibition on hyaluronidase lipoxygenase and xanthine oxidase with inhibition of 96.42 4.08 81.34 0.01and 64. 13 0.01 respectively. However the stem extract DT08/541 exhibited low activity in all assays tested. Cytotoxic effect of the extracts was investigated in vitro using Sulforhodamine B SRB assay on normal kidney Vero and normal liver WRL-68 cell lines. The results showed the median inhibitory concentration IC50 of stem and leaf extracts in both cell lines ranged from 60 ug/ml to 375 ug/ml. Since both extracts showed a IC50 value above 50 ug/ml it could be considered as non-cytotoxic. This study showed that the leaf extracts of this plant could be further explored for potential usage as remedies for inflammation treatment.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal QK99 M3S471 2008 Call Number |
| AGROVOC Term: | THYMELAEACEAE |
| AGROVOC Term: | TOXICITY |
| AGROVOC Term: | METHANOL |
| AGROVOC Term: | DAPHNE |
| AGROVOC Term: | XANTHINE OXIDASE |
| AGROVOC Term: | MALAYSIA |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:14 |
| URI: | http://webagris.upm.edu.my/id/eprint/11388 |
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