Citation
Masitah M T., . and Hapizul U I., . and Hada Masayu I D., . and Noor Rasyila M N., . and Mohd Shahidan M A., . and Rishwan H K., . and Noor Syaheera M Y., . and Iskandar H. A., . and Firmansyah J S., . and Rasadah M. A., . and Shahrul M N., . and Zamree M S.., . and Pin K Y., . and Khairul K A K., . and Mohd Faizal K., . (2009) Sinensetin and rosmaranic acid contents in different growth stages of Orthosiphon stamineus Misai Kucing. [Proceedings Paper]
Abstract
Sinensetin SEN and rosmarinic acid RA in leafand stem parts of Orthosiphon starnineus OS at different growth stages 1 2 and 3 months were quantified in order to determine the best time for harvesting purposes. An HPLC method was developed for quantification of SEN and RA. The compounds were separated using a reversed phase C18 Phenomenex column under gradient condition with methanol:water:phosphoric acid v/v/v as mobile phase. The results show that RA was the main component found in OS which was ranging from 1.31 to 1.99 of total dry leaf weight and from 0.28 to 0.32 of total dry weight stem. Concentrations of SEN ranged from 0.25 to 0.26 in leaf and 0.53 to 0.54 in stem part of OS. The results also reveal that there is no significant different of SEN concentration determined in every growth stage studied. However RA concentration on month 2 and 3 were significantly higher compared to first month. As a conclusion OS leaf contains higher concentration of RA and SEN compared to its stem and the best time to harvest OS is two months after growing.
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Abstract
Sinensetin SEN and rosmarinic acid RA in leafand stem parts of Orthosiphon starnineus OS at different growth stages 1 2 and 3 months were quantified in order to determine the best time for harvesting purposes. An HPLC method was developed for quantification of SEN and RA. The compounds were separated using a reversed phase C18 Phenomenex column under gradient condition with methanol:water:phosphoric acid v/v/v as mobile phase. The results show that RA was the main component found in OS which was ranging from 1.31 to 1.99 of total dry leaf weight and from 0.28 to 0.32 of total dry weight stem. Concentrations of SEN ranged from 0.25 to 0.26 in leaf and 0.53 to 0.54 in stem part of OS. The results also reveal that there is no significant different of SEN concentration determined in every growth stage studied. However RA concentration on month 2 and 3 were significantly higher compared to first month. As a conclusion OS leaf contains higher concentration of RA and SEN compared to its stem and the best time to harvest OS is two months after growing.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal QK99 M3S471 2008 Call Number |
| AGROVOC Term: | ROSMARINUS |
| AGROVOC Term: | ACIDITY |
| AGROVOC Term: | DRUG PLANTS |
| AGROVOC Term: | EXTRACTS |
| AGROVOC Term: | MALAYSIA |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:14 |
| URI: | http://webagris.upm.edu.my/id/eprint/11395 |
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