Bioconversion of kenaf core material into fermentable sugars by enzymatic hydrolysis

Ng. S. H. Putra Malaysia Univ., . and Luqman C. A., . and Paridah M. T. Putra Malaysia Univ., . and Rosfarizan M., . (2009) Bioconversion of kenaf core material into fermentable sugars by enzymatic hydrolysis. [Proceedings Paper]

Lignocelluloses are renewable resources for the production of fuel and chemicals. In this study bioconversion of kenaf core Hibiscus cannabinus into fermentable sugars was conducted using enzymatic hydrolysis. Kenaf core was first pretreated by i hammer milling ii physically and thermally and iii physically and chemically. The effects of the pretreatment on kenaf core lignin holocellulose and cellulose contents were determined. The pretreated kenaf core particles were enzymatically hydrolysed into fermentable sugars by cellulase from Trichoderma reesei C2730. All the pretreated kenaf core particles were incubated successively with the cellulase for 48 h at 37 C. The efficiency of the bioconversion process was determined which depended mainly on the method of pretreatment that was applied prior to hydrolysis. Kenaf core cultivar V36 from 4-month-old stems had 27.34 lignin 74.44 holocellulose and 47.40 alpha-cellulose. The physically and chemically pretreated kenaf core material resulted in lower lignin and higher cellulose contents which showed higher yield of sugars 0.28 g g-1 substrate compared with that from the physical pretreatment 0.005 g g-1 substrate.

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Abstract

Lignocelluloses are renewable resources for the production of fuel and chemicals. In this study bioconversion of kenaf core Hibiscus cannabinus into fermentable sugars was conducted using enzymatic hydrolysis. Kenaf core was first pretreated by i hammer milling ii physically and thermally and iii physically and chemically. The effects of the pretreatment on kenaf core lignin holocellulose and cellulose contents were determined. The pretreated kenaf core particles were enzymatically hydrolysed into fermentable sugars by cellulase from Trichoderma reesei C2730. All the pretreated kenaf core particles were incubated successively with the cellulase for 48 h at 37 C. The efficiency of the bioconversion process was determined which depended mainly on the method of pretreatment that was applied prior to hydrolysis. Kenaf core cultivar V36 from 4-month-old stems had 27.34 lignin 74.44 holocellulose and 47.40 alpha-cellulose. The physically and chemically pretreated kenaf core material resulted in lower lignin and higher cellulose contents which showed higher yield of sugars 0.28 g g-1 substrate compared with that from the physical pretreatment 0.005 g g-1 substrate.

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Item Type:	Proceedings Paper
Additional Information:	1 table
AGROVOC Term:	Kenaf
AGROVOC Term:	Hibiscus cannabinus
AGROVOC Term:	Bioconversion
AGROVOC Term:	Enzymatic hydrolysis
AGROVOC Term:	Lignocellulose
AGROVOC Term:	Sugar
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/11459

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