Citation
E I Elshafie, . and R A Sani rehana@vet.upm.edu.my, . and A Bashir, . and L Hassan, . and R Sharma, . (2009) Detection of Trypanosoma evansi in horses using conventional parasitological techniques and polymerase chain reaction in West Malaysia. [Proceedings Paper]
Abstract
A cross-sectional study was conducted in West Malaysia from October 2007 to August 2009 to determine the presence of Trypanosoma evansi in horses. The study was conducted in 66 stables in eight states. Horse owners were interviewed and blood samples were randomly collected. A total of 527 blood samples were obtained and microhaematocrit centrifugation technique MHCT Giemsa-stained thin blood smears and polymerase chain reactions PCR were performed. Results of this study showed an overall prevalence of0.57 3/527 using both MHCT and Giemsa-stained thin blood smears. Three positive samples were obtained from stable where the horses grazed with cattle and buffalo. Morphometric measurement was performed by measuring 50 trypanosomes from each of the three isolates. The length of a trypanosome mean SD including free flagellum was 28 2.63f1m and the mean of nuclear index was 1.32 0.18f1m. The packed cell volume PCV values of MHCT positive pies were significantly lower than the negative samples t-test 3.711; p 0.001. PCR using DNA from whole blood successfully amplified a trypanosome specific 257 bp repetitive sequence fragment in 1.14 6/527 of the samples. From this study we conclude that T.evansi prevalence is low in horses in West Malaysia and the PCV was significantly lower in positive samples.
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Abstract
A cross-sectional study was conducted in West Malaysia from October 2007 to August 2009 to determine the presence of Trypanosoma evansi in horses. The study was conducted in 66 stables in eight states. Horse owners were interviewed and blood samples were randomly collected. A total of 527 blood samples were obtained and microhaematocrit centrifugation technique MHCT Giemsa-stained thin blood smears and polymerase chain reactions PCR were performed. Results of this study showed an overall prevalence of0.57 3/527 using both MHCT and Giemsa-stained thin blood smears. Three positive samples were obtained from stable where the horses grazed with cattle and buffalo. Morphometric measurement was performed by measuring 50 trypanosomes from each of the three isolates. The length of a trypanosome mean SD including free flagellum was 28 2.63f1m and the mean of nuclear index was 1.32 0.18f1m. The packed cell volume PCV values of MHCT positive pies were significantly lower than the negative samples t-test 3.711; p 0.001. PCR using DNA from whole blood successfully amplified a trypanosome specific 257 bp repetitive sequence fragment in 1.14 6/527 of the samples. From this study we conclude that T.evansi prevalence is low in horses in West Malaysia and the PCV was significantly lower in positive samples.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | 10 ref. |
| AGROVOC Term: | Trypanosoma evansi |
| AGROVOC Term: | Horses |
| AGROVOC Term: | Polymerase chain reaction |
| AGROVOC Term: | Risk factors |
| AGROVOC Term: | Animal pathology |
| AGROVOC Term: | Blood sampling |
| AGROVOC Term: | Cross sectional analysis |
| AGROVOC Term: | West Malaysia |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:14 |
| URI: | http://webagris.upm.edu.my/id/eprint/11521 |
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