Citation
Asmah R., . and Froemming G. A. R., . and Mohd-Fadzelly A. B., . and Wan Nor I;zzah W. M. Z., . and Ibahim M. J., . (2010) Anti-cancer and anti-oxidant properties of crude extract of Eugenia aromatica l. Baill. [Proceedings Paper]
Abstract
Spices are source of antioxidants that helps to prevent the oxidative stress that leads to DNA damage and several diseases including cancer. The methanol crude extract of Eugenia aromatica L. Baill. flower or clove was investigated for its anticancer and antioxidant activities. The extract was tested against four human cancer cell lines Le. HepG2 hepatic cancer; MCF-7 hormone-dependent breast cancer; MDA-MB-231 non-hormonedependent breast cancer and HeLa cervical cancer and the normal cells 3T3 using the MTT 3-4 5-dimethylthiazol-2-yl-2 5-diphenyltetrazolium bromide assay. The methanol extract inhibited 50 cancer cell growth at concentration of 100 ug/ml. It was most effective against MCF-7. The antioxidant potential was investigated using 1 1-diphenyl-2picrylhydrazyl DPPH radical scavenging assay. High scavenging activity against DPPH radical ranging from 90 to 96 at concentrations 10 to 100 ug/ml was detected. The total phenolic content was determined by the Folin-Ciocalteau method. The result expressed as gallic acid equivalent GAEs showed that methanol extract contained 312.73 mg GAEs/g sample. The methanol extract however was not rich in f1avonoids. Results which were expressed as rutin equivalents REs showed 21.33 mg REs/g sample. In conclusion E. aromatica anti-cancer activities may be due to its anti-oxidant properties. Further study is needed to confirm this.
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Abstract
Spices are source of antioxidants that helps to prevent the oxidative stress that leads to DNA damage and several diseases including cancer. The methanol crude extract of Eugenia aromatica L. Baill. flower or clove was investigated for its anticancer and antioxidant activities. The extract was tested against four human cancer cell lines Le. HepG2 hepatic cancer; MCF-7 hormone-dependent breast cancer; MDA-MB-231 non-hormonedependent breast cancer and HeLa cervical cancer and the normal cells 3T3 using the MTT 3-4 5-dimethylthiazol-2-yl-2 5-diphenyltetrazolium bromide assay. The methanol extract inhibited 50 cancer cell growth at concentration of 100 ug/ml. It was most effective against MCF-7. The antioxidant potential was investigated using 1 1-diphenyl-2picrylhydrazyl DPPH radical scavenging assay. High scavenging activity against DPPH radical ranging from 90 to 96 at concentrations 10 to 100 ug/ml was detected. The total phenolic content was determined by the Folin-Ciocalteau method. The result expressed as gallic acid equivalent GAEs showed that methanol extract contained 312.73 mg GAEs/g sample. The methanol extract however was not rich in f1avonoids. Results which were expressed as rutin equivalents REs showed 21.33 mg REs/g sample. In conclusion E. aromatica anti-cancer activities may be due to its anti-oxidant properties. Further study is needed to confirm this.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | 2 tables; 19 ref. SB 293 S471 2010 |
| AGROVOC Term: | Eugenia aromatica |
| AGROVOC Term: | Methanol |
| AGROVOC Term: | Antioxidants |
| AGROVOC Term: | Phytochemistry |
| AGROVOC Term: | Cancer disease |
| AGROVOC Term: | Crude oil |
| AGROVOC Term: | Antineoplastic agents |
| AGROVOC Term: | Human cell culture |
| AGROVOC Term: | Spices |
| AGROVOC Term: | Oxidative stress |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:14 |
| URI: | http://webagris.upm.edu.my/id/eprint/11533 |
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