Effect of water extract of Tinospora crispa L. Hook. F. Thomson on two breast cancer cell lines MCF-7 and MDA-MB-231

Ibahim M. J., . and Wan-Nor I;zzah W. M. Z., . and Froemming G. A. R., . (2010) Effect of water extract of Tinospora crispa L. Hook. F. Thomson on two breast cancer cell lines MCF-7 and MDA-MB-231. [Proceedings Paper]

Medicinal plants have a track record for treating a variety of illnesses and they are excellent starting material for identification of new biologically active compounds. T. crispa L. Hook. F. Thomson is a recognized traditional medicinal plant used in India Indonesia Philippines and Malaysia. Yet there are numerous studies on this plant highlighting it;s anti-oxidant anti-diabetic anti-malarial and cosmetic effects but inadequate information regarding T. crispo cytotoxicity status. The objective of this study was to determine the anti-proliferative activity of water extracts of T. crispo on MCF-7 and MDA-MB-231 breast cancer cell lines where 3T3 normal fibroblast serve as control cell. Cell cytotoxicity was determined using MTT assay where tamoxifen was used as positive control. Results obtained showed that the cell viability decreased in a dose dependent manner in each cells similar to the pattern of tamoxifen. The observed IC50 values for MCF-7 MDA-MB-231 and 313 cells were 42.75 4.61Ilg/ml 46.88 1. 75 ug/ml and 65.50 4.64 ug/ml respectively. However the value obtained for 3T3 was significantly higher compared to MCF-7 and MDA-MB-231 p 0.05 df2 X2: 8.78. In conclusion water extract of T. crispo had a dose dependent anti-proliferative activity against the two breast cancer cell lines MCF-7 and MDA-MB-231 with the lowest ICso in MCF-7. Further studies are needed to elucidate the mechanism of cell death induced by T. crispa.

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Abstract

Medicinal plants have a track record for treating a variety of illnesses and they are excellent starting material for identification of new biologically active compounds. T. crispa L. Hook. F. Thomson is a recognized traditional medicinal plant used in India Indonesia Philippines and Malaysia. Yet there are numerous studies on this plant highlighting it;s anti-oxidant anti-diabetic anti-malarial and cosmetic effects but inadequate information regarding T. crispo cytotoxicity status. The objective of this study was to determine the anti-proliferative activity of water extracts of T. crispo on MCF-7 and MDA-MB-231 breast cancer cell lines where 3T3 normal fibroblast serve as control cell. Cell cytotoxicity was determined using MTT assay where tamoxifen was used as positive control. Results obtained showed that the cell viability decreased in a dose dependent manner in each cells similar to the pattern of tamoxifen. The observed IC50 values for MCF-7 MDA-MB-231 and 313 cells were 42.75 4.61Ilg/ml 46.88 1. 75 ug/ml and 65.50 4.64 ug/ml respectively. However the value obtained for 3T3 was significantly higher compared to MCF-7 and MDA-MB-231 p 0.05 df2 X2: 8.78. In conclusion water extract of T. crispo had a dose dependent anti-proliferative activity against the two breast cancer cell lines MCF-7 and MDA-MB-231 with the lowest ICso in MCF-7. Further studies are needed to elucidate the mechanism of cell death induced by T. crispa.

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Item Type:	Proceedings Paper
Additional Information:	10 ref. SB 293 S471 2010
AGROVOC Term:	Tinospora
AGROVOC Term:	Cancer disease
AGROVOC Term:	Medicinal plants
AGROVOC Term:	Breasts
AGROVOC Term:	Proliferative disorders
AGROVOC Term:	Cell-mediated cytotoxicity
AGROVOC Term:	Cell death
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/11535

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