Selection of cell lines for cell suspension culture of Artemisia annua L. of Vietnam origin

Boey P. L., . and Yahaya Z. S., . and Chan L. K., . and Ch;ng S. J., . and Tran V. M., . (2010) Selection of cell lines for cell suspension culture of Artemisia annua L. of Vietnam origin. [Proceedings Paper]

Artemisia annua L. is the main source of artemisinin which is the most efficient anti-malarial drug against the multi-drug resistant strains of Plasmodium. Due to the environmental and economical factor in vitro cell suspension culture technique was reported as one of the most efficient method for mass production of this compound. Selection of the starting material is important for establishment of cell suspension culture. Cell lines were produced from in vitro leaf explants of three A. annua clones TCl TC2 and Hi which germinated from the seeds collected from two different geographical locations in Vietnam. The growth index of the cell lines were recorded every four weeks by sub-culturing 0.5g of the callus into fresh medium for 13 subculture cycles. The cell lines were categorized into fast intermediate and slow growing groups based on the growth index of the callus. The cell lines with consistence growth index were selected for the initiation of cell suspension culture of A. annua.

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Abstract

Artemisia annua L. is the main source of artemisinin which is the most efficient anti-malarial drug against the multi-drug resistant strains of Plasmodium. Due to the environmental and economical factor in vitro cell suspension culture technique was reported as one of the most efficient method for mass production of this compound. Selection of the starting material is important for establishment of cell suspension culture. Cell lines were produced from in vitro leaf explants of three A. annua clones TCl TC2 and Hi which germinated from the seeds collected from two different geographical locations in Vietnam. The growth index of the cell lines were recorded every four weeks by sub-culturing 0.5g of the callus into fresh medium for 13 subculture cycles. The cell lines were categorized into fast intermediate and slow growing groups based on the growth index of the callus. The cell lines with consistence growth index were selected for the initiation of cell suspension culture of A. annua.

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Item Type:	Proceedings Paper
Additional Information:	6 ref. SB 293 S471 2010
AGROVOC Term:	Artemisinin
AGROVOC Term:	Callus culture
AGROVOC Term:	Artemisia annua
AGROVOC Term:	Traditional medicines
AGROVOC Term:	Suspension culture
AGROVOC Term:	Malaria
AGROVOC Term:	Vietnam Republic
AGROVOC Term:	Cell lines
AGROVOC Term:	Antimalarials
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/11548

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