Effect of cell line and subculture frequency on the establishment of cell suspension culture of Eurycoma longifolia Jack

Tan S. Y., . and Zairi J., . and Shinichi U., . and Chan L. K., . (2010) Effect of cell line and subculture frequency on the establishment of cell suspension culture of Eurycoma longifolia Jack. [Proceedings Paper]

Eurycoma longifolia Jack or more commonly known as tongkat ali is a highly prized plant that has been proven to have anti-malarial anti-pyretic anti-ulcer anxiolytic anti-cancer and aphrodisiac properties. Unfortunately the highly sought after alkaloids in tongkat ali are found in very low quantities in wild plants where a large amount of raw materials can only produce small amounts of pure extracts. Furthermore the growth and propagation rate is very slow allowing it to be harvested only after five years. Thus the number of plants in the wild is now dwindling and faces possible extinction due to over harvesting. By establishing the cell suspension culture the growth of the cells in a controlled environment can be achieved in a shorter period with production of higher quantity and quality of desired alkaloids. Various manipulations could also be made to enhance the yield in terms of cell biomass and secondary metabolite content. In this study we report on the effect of different cell lines and subculture frequency on the cell suspension culture of tongkat ali It was observed that the cell suspension cultures initiated from different cell lines originating from different trees were different in terms of cell biomass growth and physical characteristics such as colour and texture. After six subculture cycles cell biomass growth for all lines was generally stable showing that the cell suspension cultures were unaffected by subculture frequency.

Full text available from:

Abstract

Eurycoma longifolia Jack or more commonly known as tongkat ali is a highly prized plant that has been proven to have anti-malarial anti-pyretic anti-ulcer anxiolytic anti-cancer and aphrodisiac properties. Unfortunately the highly sought after alkaloids in tongkat ali are found in very low quantities in wild plants where a large amount of raw materials can only produce small amounts of pure extracts. Furthermore the growth and propagation rate is very slow allowing it to be harvested only after five years. Thus the number of plants in the wild is now dwindling and faces possible extinction due to over harvesting. By establishing the cell suspension culture the growth of the cells in a controlled environment can be achieved in a shorter period with production of higher quantity and quality of desired alkaloids. Various manipulations could also be made to enhance the yield in terms of cell biomass and secondary metabolite content. In this study we report on the effect of different cell lines and subculture frequency on the cell suspension culture of tongkat ali It was observed that the cell suspension cultures initiated from different cell lines originating from different trees were different in terms of cell biomass growth and physical characteristics such as colour and texture. After six subculture cycles cell biomass growth for all lines was generally stable showing that the cell suspension cultures were unaffected by subculture frequency.

[error in script]

Item Type:	Proceedings Paper
Additional Information:	9 ref. SB 293 S471 2010
AGROVOC Term:	Cell culture
AGROVOC Term:	Medicinal properties
AGROVOC Term:	Simaroubaceae
AGROVOC Term:	Secondary metabolites
AGROVOC Term:	Callus culture
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/11550

Actions (login required)

View Item