Citation
Harny C., . and Ajuwin L., . and Tan L. T., . and Yeo T. C., . (2011) Methods of maintaining and preserving of fungal culture to improve viability. [Proceedings Paper]
Abstract
Maintaining a viable culture is essential in any microbial research. Sarawak Biodiversity Centre has a collection of fungal cultures that is utilized for fermentations bioactive compound screening and isolation of bioactives. In this study 221 strains of uncharaterized fungi cultures were maintained using 3 preservation methods: agar slant water culture and cryostorage at -80C. They were revived and evaluated to determine their viability after one year. The results showed that 29 of the strains were preserved well in agar slant while only 8 and 2 viability were achieved for storage in water culture and cryostorage respectively. An overall viability of 72 were achieved for cultures maintained using more than one method. The strains were classified by internal transcribed spacer ITS DNA sequencing and the results showed that the viability of a subclass/order did not correlate with storage method. However using more than one preservation method is necessary to achieve higher viability in our fungal cultures.
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Abstract
Maintaining a viable culture is essential in any microbial research. Sarawak Biodiversity Centre has a collection of fungal cultures that is utilized for fermentations bioactive compound screening and isolation of bioactives. In this study 221 strains of uncharaterized fungi cultures were maintained using 3 preservation methods: agar slant water culture and cryostorage at -80C. They were revived and evaluated to determine their viability after one year. The results showed that 29 of the strains were preserved well in agar slant while only 8 and 2 viability were achieved for storage in water culture and cryostorage respectively. An overall viability of 72 were achieved for cultures maintained using more than one method. The strains were classified by internal transcribed spacer ITS DNA sequencing and the results showed that the viability of a subclass/order did not correlate with storage method. However using more than one preservation method is necessary to achieve higher viability in our fungal cultures.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | 3 ref. QH 541.5 R27 I61 2011 |
| AGROVOC Term: | Fungi |
| AGROVOC Term: | Preservation |
| AGROVOC Term: | Viability |
| AGROVOC Term: | Agar |
| AGROVOC Term: | Water culture |
| AGROVOC Term: | Malaysia |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:14 |
| URI: | http://webagris.upm.edu.my/id/eprint/11601 |
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