Diversity of actinomycetes isolated from Paya Maga Sarawak Malaysia


Citation

Ting J.Y., . and Ng Y.H., . and Tan L.T., . and Yeo T.C., . (2011) Diversity of actinomycetes isolated from Paya Maga Sarawak Malaysia. [Proceedings Paper]

Abstract

In this study actinomycetes were isolated from collected samples in an expedition to Paya Maga located at Ulu Trusan Lawas Sarawak. A total of 20 soils and 15 plant samples were collected from three different habitats: waterfall cave and forests. 51 actinomycetes strains were isolated. The majority was derived from soil samples which contributed 45 strains while the remaining 6 strains were isolated from plant samples. Among the 45 actinomycetes strains isolated from soil 18 and 2 strains were collected from waterfall and cave habitats respectively while the remaining 25 strains were isolated from forest at various elevations. For diversity study and species classification 16S ribosomal DNA were sequenced and compared to Gen Bank using Basic Local Alignment Search Tool BLAST. The results showed that the isolates are classified into four families: Streptomycetaceae Pseudonocardiaceae Streptosporangiaceae and Nocardiaceae. 51 26 strains of the isolates showed 98 maximum identity to deposit Gene bank sequences and could be classified to the genus level only. These strains could be characterized and screened for novel compounds and bioactivities.


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Abstract

In this study actinomycetes were isolated from collected samples in an expedition to Paya Maga located at Ulu Trusan Lawas Sarawak. A total of 20 soils and 15 plant samples were collected from three different habitats: waterfall cave and forests. 51 actinomycetes strains were isolated. The majority was derived from soil samples which contributed 45 strains while the remaining 6 strains were isolated from plant samples. Among the 45 actinomycetes strains isolated from soil 18 and 2 strains were collected from waterfall and cave habitats respectively while the remaining 25 strains were isolated from forest at various elevations. For diversity study and species classification 16S ribosomal DNA were sequenced and compared to Gen Bank using Basic Local Alignment Search Tool BLAST. The results showed that the isolates are classified into four families: Streptomycetaceae Pseudonocardiaceae Streptosporangiaceae and Nocardiaceae. 51 26 strains of the isolates showed 98 maximum identity to deposit Gene bank sequences and could be classified to the genus level only. These strains could be characterized and screened for novel compounds and bioactivities.

Additional Metadata

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Item Type: Proceedings Paper
Additional Information: 3 tables; 11 ref. QH 541.5 R27 I61 2011
AGROVOC Term: Actinomycetes
AGROVOC Term: Ribosomal rna
AGROVOC Term: DNA
AGROVOC Term: Streptomyces
AGROVOC Term: Sarawak
AGROVOC Term: Malaysia
Geographical Term: MALAYSIA
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 05:14
URI: http://webagris.upm.edu.my/id/eprint/11615

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