The use of molecular techniques for identification in coral reef fish genus Amphiprion

Shamala S. and Palaniappan and Siti Azizah M. N. and A. S. Othman and A. Ali. (2001) The use of molecular techniques for identification in coral reef fish genus Amphiprion. [Proceedings Paper]

The conservation of natural gene pools has become inevitable in order for successful sustenance of species, especially those endangered. Commercial exploitation may rapidly diminish genetic variation in populations. Besides the impacts on a specific species, damage may also be caused towards the economic potential of the species and the ecosystem at large. Identification of the species present is vital in any conservation programme. Classical taxonomic methods have been difficult to employ because of the ambiguity in results obtained. The trend has now moved on molecular techniques that are more amplified and clarified. However integration of both methods has been proven to produce outstanding results. The use of molecular techniques has become very important as a method to sustain biodiversity of ecosystems and in this case, coral reefs. Anemone fishes (genus Amphiprion) are members of the order Perciformes, with approximately 27 species. Colour pattern is the most important feature for identifying an anemone fish in the field. Correct identification has been documented to be difficult because of the presence of common geographic colour variants that occur in some wide- ranging species. Furthermore, juvenile identification is difficult due to great similarities between species. Hence, DNA sequencing methods along with morphological characteristics have been employed to determine intraspecies variation in anemone fishes. Incorporated with other fingerprinting methodology, genetic variation amongst populations can be determined. Conservation for species-specific conditions is made easier and misidentification of the migratory species can be overcome.

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Abstract

The conservation of natural gene pools has become inevitable in order for successful sustenance of species, especially those endangered. Commercial exploitation may rapidly diminish genetic variation in populations. Besides the impacts on a specific species, damage may also be caused towards the economic potential of the species and the ecosystem at large. Identification of the species present is vital in any conservation programme. Classical taxonomic methods have been difficult to employ because of the ambiguity in results obtained. The trend has now moved on molecular techniques that are more amplified and clarified. However integration of both methods has been proven to produce outstanding results. The use of molecular techniques has become very important as a method to sustain biodiversity of ecosystems and in this case, coral reefs. Anemone fishes (genus Amphiprion) are members of the order Perciformes, with approximately 27 species. Colour pattern is the most important feature for identifying an anemone fish in the field. Correct identification has been documented to be difficult because of the presence of common geographic colour variants that occur in some wide- ranging species. Furthermore, juvenile identification is difficult due to great similarities between species. Hence, DNA sequencing methods along with morphological characteristics have been employed to determine intraspecies variation in anemone fishes. Incorporated with other fingerprinting methodology, genetic variation amongst populations can be determined. Conservation for species-specific conditions is made easier and misidentification of the migratory species can be overcome.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad, Universiti Putra Malaysia, 43000 UPM Serdang, Malaysia.Call Number QH75 A1I61 2000
AGROVOC Term:	molecular characterization
AGROVOC Term:	genetic variation
AGROVOC Term:	identification
AGROVOC Term:	coral reefs
AGROVOC Term:	fish
AGROVOC Term:	Pomacentridae
AGROVOC Term:	morphology
AGROVOC Term:	taxonomy
AGROVOC Term:	molecular genetics
Geographical Term:	Malaysia
Uncontrolled Keywords:	Amphiprion, coral reef fish
Depositing User:	Ms. Azariah Hashim
Date Deposited:	09 Sep 2024 06:58
Last Modified:	09 Sep 2024 06:58
URI:	http://webagris.upm.edu.my/id/eprint/1165

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