Citation
Mohamad Ramlan Mohamed Salleh, . and Parveen Jamal, . and Rohani Salleh, . and Haslinda Hasim, . (2006) Effect of isolation time and the sonicator;s amplitude on the isolation of GSH from yeast by ultrasonic method. [Proceedings Paper]
Abstract
The effect of isolation time and amplitude of the sonicator on the isolation of GSH from baker yeast cells were studied. The yeast cells were disrupted by Ultrasonic Method. The disrupted cells solution was then centrifuged at 12 000 rpm for 20 minutes and the GSH content was analyzed by Spectrophotometer at 412 nm. The results obtained showed that the optimum value of the isolation time and the amplitude of the sonicator in isolating the optimum value of GSH was 15 sec and 84 m 40 respectively where 18.13 mol/ml GSH was isolated.
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Abstract
The effect of isolation time and amplitude of the sonicator on the isolation of GSH from baker yeast cells were studied. The yeast cells were disrupted by Ultrasonic Method. The disrupted cells solution was then centrifuged at 12 000 rpm for 20 minutes and the GSH content was analyzed by Spectrophotometer at 412 nm. The results obtained showed that the optimum value of the isolation time and the amplitude of the sonicator in isolating the optimum value of GSH was 15 sec and 84 m 40 respectively where 18.13 mol/ml GSH was isolated.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. RS180 M3I61 2005 Call Number |
| AGROVOC Term: | Isolation |
| AGROVOC Term: | Yeasts |
| AGROVOC Term: | Ultrasonics |
| AGROVOC Term: | Spectrophotometers |
| AGROVOC Term: | Glutathione |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:14 |
| URI: | http://webagris.upm.edu.my/id/eprint/11715 |
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