Studies on the effects of honey on oxidative status and embryonic development of stress-induced mice

Siti Aishah B., . and Zaiton Z., . and Rajikin M. H., . and Kamaruddin M. Y., . (2006) Studies on the effects of honey on oxidative status and embryonic development of stress-induced mice. [Proceedings Paper]

The purpose of this study was to determine the effects of honey on oxidative status and development of preimplantation embryo in stress-induced female ICR strain mice. Honey was collected from a pineapple farm in Batu Pahat Johor. 32 animals were divided into 4 groups; control group distilled water orally honey alone 2 mg/kg body weight orally stress group water immersion stress in 22 C 1/2 hour and the fourth group was the stress-induced group with oral supplementation of honey 2 mg/kg body weight. Significantly p 0.05 higher levels of lipid peroxidation MDA levels were found in the plasma of the stress group 9.56370.9940 as compared to the control group 5.2920O.8657 whereas those supplemented with honey showed lower level of plasma MDA 5.05330.2474. The detrimental effects of stress and those supplemented with honey groups again were consistent with the high and low levels of plasma MDA respectively. Research on preimplantation embryos revealed that out of 366 embryos retrieved; only 60 16.39 were produced from stress group mice. Furthermore out of 60 embryos only 6.7 attained hatching blastocyst stage. Contrary to this honey protected the detrimental effects of stress in which out of 85 embryos retrieved; 21 attained hatched blastocysts. The role of honey was even clearer when the group was given honey alone 34 of the developed blastocysts hatched. In summary it could be concluded that the impairment of reproductive functions in the stress animals was probably due to the enhancement of lipid peroxidation reflected by increase in the level of plasma MDA concentration. Honey acted as an antioxidant agent which protected against lipid peroxidation and therefore improved the reproductive functions of the animals.

Full text available from:

Abstract

The purpose of this study was to determine the effects of honey on oxidative status and development of preimplantation embryo in stress-induced female ICR strain mice. Honey was collected from a pineapple farm in Batu Pahat Johor. 32 animals were divided into 4 groups; control group distilled water orally honey alone 2 mg/kg body weight orally stress group water immersion stress in 22 C 1/2 hour and the fourth group was the stress-induced group with oral supplementation of honey 2 mg/kg body weight. Significantly p 0.05 higher levels of lipid peroxidation MDA levels were found in the plasma of the stress group 9.56370.9940 as compared to the control group 5.2920O.8657 whereas those supplemented with honey showed lower level of plasma MDA 5.05330.2474. The detrimental effects of stress and those supplemented with honey groups again were consistent with the high and low levels of plasma MDA respectively. Research on preimplantation embryos revealed that out of 366 embryos retrieved; only 60 16.39 were produced from stress group mice. Furthermore out of 60 embryos only 6.7 attained hatching blastocyst stage. Contrary to this honey protected the detrimental effects of stress in which out of 85 embryos retrieved; 21 attained hatched blastocysts. The role of honey was even clearer when the group was given honey alone 34 of the developed blastocysts hatched. In summary it could be concluded that the impairment of reproductive functions in the stress animals was probably due to the enhancement of lipid peroxidation reflected by increase in the level of plasma MDA concentration. Honey acted as an antioxidant agent which protected against lipid peroxidation and therefore improved the reproductive functions of the animals.

[error in script]

Item Type:	Proceedings Paper
Additional Information:	Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. RS180 M3I61 2005 Call Number
AGROVOC Term:	Honey
AGROVOC Term:	Oxidative stability
AGROVOC Term:	Embryonic development
AGROVOC Term:	Stress
AGROVOC Term:	Mice
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/11716

Actions (login required)

View Item