Flocculating activity of bioflocculant producing bacteria isolated from Closed Drainage System CDS at the Prai Industrial zone Penang Malaysia

R. Hazana, . and I. Norli, . and M. H. Ibrahim, . and A. Fazilah, . (2008) Flocculating activity of bioflocculant producing bacteria isolated from Closed Drainage System CDS at the Prai Industrial zone Penang Malaysia. [Proceedings Paper]

Bioflocculant is a polymer produced by microorganisms during their growth with their flocculating activity being dependent on the characteristics of the flocculants. Bioflocculant producing bacteria were isolated from wastewater and sediments of Close Drainage Systems CDS located at the Prai industrial area. Polyglutamic Acid PGA medium was used as a selective medium to grow the bioflocculant producing bacteria. The cultures were incubated at 30to 50 DC for 43 to 77 hours. The highest flocculating activity was obtained by measuring the optical density OD at 550nm of the kaolin suspension hydrated aluminum silicate after the addition of culture broth. More than 300 colonies were screened and isolated from microbial source samples based on their ability to grow on Polyglutamic acid PGA medium. Two types of bacterial with flocculation rate of 97.01 and 96.82 were identified as Sphingomonas paucimobilis and Staphylococcus lentus respectively.

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Abstract

Bioflocculant is a polymer produced by microorganisms during their growth with their flocculating activity being dependent on the characteristics of the flocculants. Bioflocculant producing bacteria were isolated from wastewater and sediments of Close Drainage Systems CDS located at the Prai industrial area. Polyglutamic Acid PGA medium was used as a selective medium to grow the bioflocculant producing bacteria. The cultures were incubated at 30to 50 DC for 43 to 77 hours. The highest flocculating activity was obtained by measuring the optical density OD at 550nm of the kaolin suspension hydrated aluminum silicate after the addition of culture broth. More than 300 colonies were screened and isolated from microbial source samples based on their ability to grow on Polyglutamic acid PGA medium. Two types of bacterial with flocculation rate of 97.01 and 96.82 were identified as Sphingomonas paucimobilis and Staphylococcus lentus respectively.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. GE90 M3I61 2008 Call Number
AGROVOC Term:	Flocculation
AGROVOC Term:	Staphylococcus
AGROVOC Term:	Bacteria
AGROVOC Term:	Drainage systems
AGROVOC Term:	Polymers
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/11809

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