Utilization of isozyme marken for evaluation of tropical fruit and nut germplasm at the USDA/ARS clonal germplasm repository

Zee Francis, . and Malli Aradhya, . and Richard Manshardt, . and Sui Li, . (1996) Utilization of isozyme marken for evaluation of tropical fruit and nut germplasm at the USDA/ARS clonal germplasm repository. [Proceedings Paper]

The major responsibilities of a germplasm repository are: 1 to establish the genetic identity fingerprint of accessions in the collection; 2 to-evaluate classify and manage the collection by preserving the level and patterns of genetic diversity; and 3 to develop strategies for future collections to enhance the gene pool. Evaluation of the existing collections is a prerequisite to address these objective. Morphological traits are not stable across environments and developmental stages especially in tree crops and hence are not suitable for genetic diversity assessment or identification. Isozyme markers are stable and extensively used in the identification evaluation and classification of genetic resources crop plants. The utility of isozymes as genetic markers is generally attributed to their ubiquity in plant tissues and organs frequent polymorphism codominance Mendelian inheritance simple rapid and inexpensive assay. Isozymedata lends itself to population genetic and multivariate analyses to quantify genetic variation and to group germplasm accession based on their multivariate relationships. Not all enzymes have the required genetic basis for using them in the analysis of genetic diversity in the germplasm collections however they can be used in fingerprinting and classifying germplasm. At the National Clonal Germplasm Repository NCGR USDA-ARS Hilo Hawaii isozyme electrophoresis is routinely used to evaluate germplasm. The data are subjected to a number of statistical analyses techniques to assess the genetic diversity and to classify the germplasm to reveal the multivariate genetic relationships between accessions groups and to species. The utility of isozyme markers in unraveling the genetic diversity and relationships within and between cultivated and wild species of lychee macadamia nut carambola pili nut pineapple and rambutan are discussed in this poster.

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Abstract

The major responsibilities of a germplasm repository are: 1 to establish the genetic identity fingerprint of accessions in the collection; 2 to-evaluate classify and manage the collection by preserving the level and patterns of genetic diversity; and 3 to develop strategies for future collections to enhance the gene pool. Evaluation of the existing collections is a prerequisite to address these objective. Morphological traits are not stable across environments and developmental stages especially in tree crops and hence are not suitable for genetic diversity assessment or identification. Isozyme markers are stable and extensively used in the identification evaluation and classification of genetic resources crop plants. The utility of isozymes as genetic markers is generally attributed to their ubiquity in plant tissues and organs frequent polymorphism codominance Mendelian inheritance simple rapid and inexpensive assay. Isozymedata lends itself to population genetic and multivariate analyses to quantify genetic variation and to group germplasm accession based on their multivariate relationships. Not all enzymes have the required genetic basis for using them in the analysis of genetic diversity in the germplasm collections however they can be used in fingerprinting and classifying germplasm. At the National Clonal Germplasm Repository NCGR USDA-ARS Hilo Hawaii isozyme electrophoresis is routinely used to evaluate germplasm. The data are subjected to a number of statistical analyses techniques to assess the genetic diversity and to classify the germplasm to reveal the multivariate genetic relationships between accessions groups and to species. The utility of isozyme markers in unraveling the genetic diversity and relationships within and between cultivated and wild species of lychee macadamia nut carambola pili nut pineapple and rambutan are discussed in this poster.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal SB 359 I61 1996 Vol. 1 Call Number
AGROVOC Term:	Isozymes
AGROVOC Term:	Tropical fruits
AGROVOC Term:	Germplasm
AGROVOC Term:	Clones
AGROVOC Term:	Genetics
AGROVOC Term:	Genetic diversity within species
AGROVOC Term:	Tree crops
AGROVOC Term:	Nonmendelian inheritance
AGROVOC Term:	Identification
AGROVOC Term:	Electrophoresis
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/11921

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