Current techniques in the analysis of fibre of fruit tissues

Koh T. H., . and Melton L. D., . (1996) Current techniques in the analysis of fibre of fruit tissues. [Proceedings Paper]

Changes in the cell wall composition of fruit during ripening account for alterations in textural properties which can lead to both desirable and undesirable postharvest qualities. To further understand this mechanism during the ripening period it is important to establish which cell wall polymers are altered significantly as softening develops and determine what types of structural changes are occurring. This paper describes current techniques used in our research laboratory in the analysis of fruit cell walls in relation to ripening of fruit such as papaya paw paw and strawberries. Cell wall materials are isolated after treating frozen fruit powder with phenol/HEPES 4: 1 w/v at pH 6.5. The isolate is then sequentially fractionated using a sequence of solvents with increasing solvation strength. Fractions containing polysaccharides are then studied in detail using gas chromatography. Solid-state l3C cross polarisation magic-angle spinning nuclear magnetic resonance of the isolated fruit cell wall material is also used to explore the three-dimensional arrangement of polysaccharides in the cell walls.

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Abstract

Changes in the cell wall composition of fruit during ripening account for alterations in textural properties which can lead to both desirable and undesirable postharvest qualities. To further understand this mechanism during the ripening period it is important to establish which cell wall polymers are altered significantly as softening develops and determine what types of structural changes are occurring. This paper describes current techniques used in our research laboratory in the analysis of fruit cell walls in relation to ripening of fruit such as papaya paw paw and strawberries. Cell wall materials are isolated after treating frozen fruit powder with phenol/HEPES 4: 1 w/v at pH 6.5. The isolate is then sequentially fractionated using a sequence of solvents with increasing solvation strength. Fractions containing polysaccharides are then studied in detail using gas chromatography. Solid-state l3C cross polarisation magic-angle spinning nuclear magnetic resonance of the isolated fruit cell wall material is also used to explore the three-dimensional arrangement of polysaccharides in the cell walls.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal SB 359 I61 1996 Vol. 2 Call Number
AGROVOC Term:	Fruits
AGROVOC Term:	Fibres
AGROVOC Term:	Cell walls
AGROVOC Term:	Ripening
AGROVOC Term:	Texture
AGROVOC Term:	Postharvest activities
AGROVOC Term:	Polymers
AGROVOC Term:	Papayas
AGROVOC Term:	Strawberries
AGROVOC Term:	Gas chromatography
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/11990

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