Identification of steroidogenic acute regulatory sTAR gene in Malaysian indigenous sheep using PCR based substractive hybridization method

Ainu-Husna M. S. S, . and Azlina Azma I. A., . and Roziatul Erin A. R., . (2004) Identification of steroidogenic acute regulatory sTAR gene in Malaysian indigenous sheep using PCR based substractive hybridization method. [Proceedings Paper]

A substractive complementary DNA eDNA library based on polymerase chain reaction was constructed to identify differential genes in Malaysian Indigenous Malin sheep with high twinning rate. Five clones were isolated and sequenced. From the five clones one clone had an insert that has a 91 homology with Bos taurus Steroidogenic Acute Regulatory sTAR gene. sTAR gene which encoded for Steroidogenic Acute Regulatory sTAR protein is essential for adrenal and gonadal steridogenesis. Absence of this gene was shown to cause several impairment to ovarian follicular development and/or ovulation rate of sexually matured 8 weeks old mice. This gene may have influenced the ovulation rate of animals with high twinning rate in Malin sheep population thus causing increased number of litter at a single birth.

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Abstract

A substractive complementary DNA eDNA library based on polymerase chain reaction was constructed to identify differential genes in Malaysian Indigenous Malin sheep with high twinning rate. Five clones were isolated and sequenced. From the five clones one clone had an insert that has a 91 homology with Bos taurus Steroidogenic Acute Regulatory sTAR gene. sTAR gene which encoded for Steroidogenic Acute Regulatory sTAR protein is essential for adrenal and gonadal steridogenesis. Absence of this gene was shown to cause several impairment to ovarian follicular development and/or ovulation rate of sexually matured 8 weeks old mice. This gene may have influenced the ovulation rate of animals with high twinning rate in Malin sheep population thus causing increased number of litter at a single birth.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia HD9410.1 A111 2004 vol.3 Call Number
AGROVOC Term:	Sheep
AGROVOC Term:	Acute disease
AGROVOC Term:	Genes
AGROVOC Term:	PCR
AGROVOC Term:	Hybridization
AGROVOC Term:	RNA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/12035

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