Production of bovine blastocyst after serum supplement at morula stage

Nor Fadillah A., . and Wan Khadijah W. E., . and Abdullah R. B., . (2004) Production of bovine blastocyst after serum supplement at morula stage. [Proceedings Paper]

This preliminary study of in vitro maturation fertilization and culture IVMFC was carried out as a comparative study on the effect of serum addition at morula stage embryos. Ovaries from slaughtered cattle were sliced to obtain oocytes. Recovered oocytes were matured in vitro in TCM 199 medium for 24 hours in CO2 incubator at 39C. Matured oocytes were fertilized with frozen-thawed sperm in BO medium for 7 hours and cultured in SOF medium for further development. At morula stage a proportion of embryos were added with sterile serum and others were developed without additional serum. Better performance of embryo development at blastocyst stage was achieved when embryos were added with serum at morula stage compared to embryos without additional serum. Percentages of embryo development with and without serum addition were 59.5 and 59.6; 48.7 and 50.2; 39.3 and 38.5; 29.8 and 28.8; 10.6 and 1.0 at 2- 4- 8-cell morula and early blastocyst stage respectively. This experiment showed that addition of serum to the embryos at morula stage can enhanced the rate of blastocyst higher than without serum.

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Abstract

This preliminary study of in vitro maturation fertilization and culture IVMFC was carried out as a comparative study on the effect of serum addition at morula stage embryos. Ovaries from slaughtered cattle were sliced to obtain oocytes. Recovered oocytes were matured in vitro in TCM 199 medium for 24 hours in CO2 incubator at 39C. Matured oocytes were fertilized with frozen-thawed sperm in BO medium for 7 hours and cultured in SOF medium for further development. At morula stage a proportion of embryos were added with sterile serum and others were developed without additional serum. Better performance of embryo development at blastocyst stage was achieved when embryos were added with serum at morula stage compared to embryos without additional serum. Percentages of embryo development with and without serum addition were 59.5 and 59.6; 48.7 and 50.2; 39.3 and 38.5; 29.8 and 28.8; 10.6 and 1.0 at 2- 4- 8-cell morula and early blastocyst stage respectively. This experiment showed that addition of serum to the embryos at morula stage can enhanced the rate of blastocyst higher than without serum.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia HD9410.1 A111 2004 vol.3 Call Number
AGROVOC Term:	Bovines
AGROVOC Term:	Blastocysts
AGROVOC Term:	In vitro culture
AGROVOC Term:	Immune serum
AGROVOC Term:	Embryonic development
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/12041

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