Bovine embryo sexing by fluorescence in situ hybridization technique

Ha H. B., . and Cho E. J., . and Sohn S. H., . and Han M. S., . and Park H. S., . (2004) Bovine embryo sexing by fluorescence in situ hybridization technique. [Proceedings Paper]

Sex of bovine embryos was determined by fluorescence in situ hybridization FISH technique with a bovine V-specific probe a partial btDYZ-l. To evaluate Y chromosome specificity of the FISH probe metaphase spreads of whole embryos were prepared and tested. A male-specific signal was detected on 100 of Y chromosome bearing metaphase specimens. Using the FISH technique with a bovine Y-specific probe 71 biopsied embryos which were from 8 cells to blastocysts were analyzed. Observing the presence of the Y-probe signal on blastomeres 31 embryos were predicted as male and 40 embryos as female.

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Abstract

Sex of bovine embryos was determined by fluorescence in situ hybridization FISH technique with a bovine V-specific probe a partial btDYZ-l. To evaluate Y chromosome specificity of the FISH probe metaphase spreads of whole embryos were prepared and tested. A male-specific signal was detected on 100 of Y chromosome bearing metaphase specimens. Using the FISH technique with a bovine Y-specific probe 71 biopsied embryos which were from 8 cells to blastocysts were analyzed. Observing the presence of the Y-probe signal on blastomeres 31 embryos were predicted as male and 40 embryos as female.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia HD9410.1 A111 2004 vol.3 Call Number
AGROVOC Term:	Bovines
AGROVOC Term:	Embryo sexing
AGROVOC Term:	Fluorescence
AGROVOC Term:	Hybridization
AGROVOC Term:	Biopsy
AGROVOC Term:	Chromosomes
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/12048

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