Expression of tissue-type plasminogen activator in the porcine urine of transgenic swine


Citation

Park C. G., . and Lee H. T., . and Lee H. G., . and Lee P. Y., . and Han J. H., . and Lee Y. K., . and Chang W. K., . and Jeon I. S., . and Lee C. H., . and Seong H. H., . and Kim S. W., . and Min K. S., . and Park J. K., . (2004) Expression of tissue-type plasminogen activator in the porcine urine of transgenic swine. [Proceedings Paper]

Abstract

Tissue plasminogen activator tPA play important roles in the brain after excitotoxic injury. This study was conducted to produce transgenic pig harboring human tissue plasminogene activator htPA gene. Recombinent htPA rhtPA genes containing mouse uroplakin promoter mUPIl were prepared for microinjection and testified the expression level of htPA protein from the Chinese hamster ovary CHO cell lines before DNA microinjection into the porcine pronuclei. Concentration of htPA expression from the six cell lines all of CHO cells were an average of212.4 ng/mI. Reconstructed DNA to use the CHO cells were microinjected into the pronuclei of in vivo derived porcine embryos. The 1 246 embryos microinjected with mouse uroplakin promoter-tPA were transferred to 55 recipients. Forty offspring from 6 sows were delivered. We analysed the transgenes with PCR methods from 40 piglet tails DNA and two of them were detected as transgenic pigs. This result show the potential of purified rh-tPA derived from transgenic pig urine could be used for the treatment of thrombus in humans.


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Abstract

Tissue plasminogen activator tPA play important roles in the brain after excitotoxic injury. This study was conducted to produce transgenic pig harboring human tissue plasminogene activator htPA gene. Recombinent htPA rhtPA genes containing mouse uroplakin promoter mUPIl were prepared for microinjection and testified the expression level of htPA protein from the Chinese hamster ovary CHO cell lines before DNA microinjection into the porcine pronuclei. Concentration of htPA expression from the six cell lines all of CHO cells were an average of212.4 ng/mI. Reconstructed DNA to use the CHO cells were microinjected into the pronuclei of in vivo derived porcine embryos. The 1 246 embryos microinjected with mouse uroplakin promoter-tPA were transferred to 55 recipients. Forty offspring from 6 sows were delivered. We analysed the transgenes with PCR methods from 40 piglet tails DNA and two of them were detected as transgenic pigs. This result show the potential of purified rh-tPA derived from transgenic pig urine could be used for the treatment of thrombus in humans.

Additional Metadata

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Item Type: Proceedings Paper
Additional Information: Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia HD9410.1 A111 2004 vol.3 Call Number
AGROVOC Term: Pigs
AGROVOC Term: Tissue
AGROVOC Term: Plasminogen activator
AGROVOC Term: Transgenic animals
AGROVOC Term: Recombinant DNA
AGROVOC Term: Cell culture
Geographical Term: MALAYSIA
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 05:14
URI: http://webagris.upm.edu.my/id/eprint/12078

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