Molecular analysis of CSN3 alleles in the indigenous Iranian Sarabi cattle using PCR-RFLP

Tahvildarzadeh A., . and Shoja J., . and Toorchi M., . (2004) Molecular analysis of CSN3 alleles in the indigenous Iranian Sarabi cattle using PCR-RFLP. [Proceedings Paper]

Data on genetic differences at CSN3 K -casein in the indigenous Iranian Sarabi cattle n 66 and Holstein HF n 102 were analysed using a DNA based typing method. Gene frequencies for A and B alleles in Holstein and Sarabi cattle breeds were estimated to be 0.8284 0.0265 0.1716 0.0265 and 0.7652 0.0361 0.2348 0.0361 respectively. Comparison of allele frequencies in this study indicated that there was no significant difference p0.05 between observed and expected frequencies in both Sarabi and Holstein populations. The observed heterozygosity for Sarabi and Holstein were 0.3484 and 0.2843 respectively. The estimated gene diversity from sum of squares of allele frequency represented for Sarabi and Holstein were 0.3593 and 0.2843 respectively. Comparison of allele frequencies of this study with those of other cattle breeds indicated that the two breeds employed in the present study were significantly different from both Jersey and Brown Swiss breeds.

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Abstract

Data on genetic differences at CSN3 K -casein in the indigenous Iranian Sarabi cattle n 66 and Holstein HF n 102 were analysed using a DNA based typing method. Gene frequencies for A and B alleles in Holstein and Sarabi cattle breeds were estimated to be 0.8284 0.0265 0.1716 0.0265 and 0.7652 0.0361 0.2348 0.0361 respectively. Comparison of allele frequencies in this study indicated that there was no significant difference p0.05 between observed and expected frequencies in both Sarabi and Holstein populations. The observed heterozygosity for Sarabi and Holstein were 0.3484 and 0.2843 respectively. The estimated gene diversity from sum of squares of allele frequency represented for Sarabi and Holstein were 0.3593 and 0.2843 respectively. Comparison of allele frequencies of this study with those of other cattle breeds indicated that the two breeds employed in the present study were significantly different from both Jersey and Brown Swiss breeds.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia HD9410.1 A111 2004 vol.3 Call Number
AGROVOC Term:	Dairy cattle
AGROVOC Term:	Alleles
AGROVOC Term:	Genetic differences
AGROVOC Term:	Heterozygotes
AGROVOC Term:	Breeding
AGROVOC Term:	PCR
AGROVOC Term:	Genetic variation
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/12082

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