Citation
Sarbini S. R., . and Ong K. H., . and Illias M. R., . and Jahim J. M., . and Bakar F. D . A., . and Bong C. F. J., . and Mahadi N. M., . and Hii M. H. l., . and Hassan O., . and King P. J. H., . and Said M., . and Murad A. A. M., . and Rabu A., . (2014) Isolation and characterization of endoglucanase microbes residing in the termite Coptotermes Curvignathusgut. [Proceedings Paper]
Abstract
Termites can consume up to 2 billion economy value of wooden material per year thus their voracious appetite for lignocellulolytic material become an interesting gold mine for robust cellulolytic bacteria and enzymes. There are more than 2300species of termites and Coptotermes curvignathus is one of the very few destructive termite species that can attacks living trees. This indicates the intestinal microbiome of C. curvignathus may excrete unique lignocellulolytic enzymes thatcan overcome the defense mechanism of healthy living plant. In this study we have managed to identify four endoglucanase producing bacteria isolates from C. curvignathusgut which hydrolyzes the cellulose polymers into cellobiose. Four Bacillus spp. were identified to produce endoglucanase of molecular size 10 kDa to 17 kDa. The molecular size of these enzymes is significant smaller than the reported 41 kDA and 42kDa of endoglucanase produced by Reticulitermessperatus. Specific functional characteristics of these enzymes will be further investigated for the development of robust industrial enzymes for consolidation of biorefinery process.
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Abstract
Termites can consume up to 2 billion economy value of wooden material per year thus their voracious appetite for lignocellulolytic material become an interesting gold mine for robust cellulolytic bacteria and enzymes. There are more than 2300species of termites and Coptotermes curvignathus is one of the very few destructive termite species that can attacks living trees. This indicates the intestinal microbiome of C. curvignathus may excrete unique lignocellulolytic enzymes thatcan overcome the defense mechanism of healthy living plant. In this study we have managed to identify four endoglucanase producing bacteria isolates from C. curvignathusgut which hydrolyzes the cellulose polymers into cellobiose. Four Bacillus spp. were identified to produce endoglucanase of molecular size 10 kDa to 17 kDa. The molecular size of these enzymes is significant smaller than the reported 41 kDA and 42kDa of endoglucanase produced by Reticulitermessperatus. Specific functional characteristics of these enzymes will be further investigated for the development of robust industrial enzymes for consolidation of biorefinery process.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia TP339 B615 Call Number |
| AGROVOC Term: | Coptotermes |
| AGROVOC Term: | Termites |
| AGROVOC Term: | Glucans |
| AGROVOC Term: | Microbes |
| AGROVOC Term: | Isolation |
| AGROVOC Term: | Enzymes |
| AGROVOC Term: | Bacteria |
| AGROVOC Term: | Pests of plants |
| AGROVOC Term: | Cellulolytic microorganisms |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:14 |
| URI: | http://webagris.upm.edu.my/id/eprint/12096 |
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