Can carotino red palm oil activate/deactive p38 mapk and pkb/akt associated with cardiac protection

Rooyen, Jacques van and Esterhuyse, Johan and Engelbrecht, Anna-Mart and Toit, Eugene F. Du. (2005) Can carotino red palm oil activate/deactive p38 mapk and pkb/akt associated with cardiac protection. [Proceedings Paper]

Introduction: It has been shown that dietary red palm oil supplementation improves reperfusion function. The protection has been linked to a decrease in CAMP and increase in cGMP during ischaemia in isolated perfused rat hearts (Esterhuyse et al., 2005). However, we have reason to believe that other mechanisms may also play a role during reperfusion. Therefore, our aim was to examine the ischaemc/reperfusion regulation of mitogen-activated protein kinase (MAPKS) and PKB/Akt in the dietary presence and absence of red palm oil. Methods: Wistar rats were fed a control diet or control diet plus 7g RPO/kg diet for 6 weeks. Hearts were excised and mounted on an isolated working heart perfusion apparatus. Cardiac function was measured before and after hearts was subjected to 25 minutes of total global ischaemia. Hearts subjected to the same conditions were freeze- clamped and used to characterize the degree of phosphorylation of MAPKs and PKB/Akt during ischaemia and reperfusion. Results: Dietary RPO supplementation significantly improved aortic output recovery associated with a significant increase in p38 and PKB/Akt phosphorylation during reperfusion when compared with control hearts. JNK phosphorylation was significantly decreased with a subsequent attenuation of apoptosis in the RPO supplemented group during reperfusion. Conclusion: Our results suggest that dietary RPO supplementation caused differential phosphorylation of MAPKs and PKB/Akt during ischaemia/reperfusion-induced injury. These changes in phosphorylation were associated with improved functional recovery. Interestingly, phosphorylation occurred during reperfusion and not during ischaemia.

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Abstract

Introduction: It has been shown that dietary red palm oil supplementation improves reperfusion function. The protection has been linked to a decrease in CAMP and increase in cGMP during ischaemia in isolated perfused rat hearts (Esterhuyse et al., 2005). However, we have reason to believe that other mechanisms may also play a role during reperfusion. Therefore, our aim was to examine the ischaemc/reperfusion regulation of mitogen-activated protein kinase (MAPKS) and PKB/Akt in the dietary presence and absence of red palm oil. Methods: Wistar rats were fed a control diet or control diet plus 7g RPO/kg diet for 6 weeks. Hearts were excised and mounted on an isolated working heart perfusion apparatus. Cardiac function was measured before and after hearts was subjected to 25 minutes of total global ischaemia. Hearts subjected to the same conditions were freeze- clamped and used to characterize the degree of phosphorylation of MAPKs and PKB/Akt during ischaemia and reperfusion. Results: Dietary RPO supplementation significantly improved aortic output recovery associated with a significant increase in p38 and PKB/Akt phosphorylation during reperfusion when compared with control hearts. JNK phosphorylation was significantly decreased with a subsequent attenuation of apoptosis in the RPO supplemented group during reperfusion. Conclusion: Our results suggest that dietary RPO supplementation caused differential phosphorylation of MAPKs and PKB/Akt during ischaemia/reperfusion-induced injury. These changes in phosphorylation were associated with improved functional recovery. Interestingly, phosphorylation occurred during reperfusion and not during ischaemia.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. TP684 P3I61 2005 Call Number
AGROVOC Term:	palm oils
AGROVOC Term:	plant oils
AGROVOC Term:	functional foods
AGROVOC Term:	heart diseases
AGROVOC Term:	experimental design
AGROVOC Term:	data analysis
AGROVOC Term:	carotenoids
AGROVOC Term:	tocotrienols
AGROVOC Term:	oxidative stress
Geographical Term:	South Africa
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	01 Nov 2025 07:22
Last Modified:	10 Nov 2025 01:04
URI:	http://webagris.upm.edu.my/id/eprint/1216

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