Citation
Muhammad Rashdan M., . and Ruslan Abdullah, . (1999) Agrobacterium-mediated transformation of oil palm. [Proceedings Paper]
Abstract
Immature oil palm embryos were independently co-cultivated with two Agrobacterium tumefaciens LBA4404 strains. Each strain carries either a super binary vector pTOK233 or an ordinary vector pCAMBIA1301 harboring both the hygromycin phosphotransferase hpt and -glucuronidase gus genes. Pre-treatment of target tissues in 2 4-D-containing media prior to co-cultivation was shown to enhance Agrobacterium infections. Between 11.1-86.0 of randomly selected germinated immature embryos showed transient GUS activity. Of these 14.3-64.3 were resistant to hygromycin upon selection on hygromycin-containingmedium. Evidence of successful gene transfer was demonstrated using PCR-Southem hybridization.
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Abstract
Immature oil palm embryos were independently co-cultivated with two Agrobacterium tumefaciens LBA4404 strains. Each strain carries either a super binary vector pTOK233 or an ordinary vector pCAMBIA1301 harboring both the hygromycin phosphotransferase hpt and -glucuronidase gus genes. Pre-treatment of target tissues in 2 4-D-containing media prior to co-cultivation was shown to enhance Agrobacterium infections. Between 11.1-86.0 of randomly selected germinated immature embryos showed transient GUS activity. Of these 14.3-64.3 were resistant to hygromycin upon selection on hygromycin-containingmedium. Evidence of successful gene transfer was demonstrated using PCR-Southem hybridization.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. Call number mal SB 950 I61 1999 |
| AGROVOC Term: | Agrobacterium |
| AGROVOC Term: | Oil palm |
| AGROVOC Term: | Dicotyledons |
| AGROVOC Term: | Plant embryos |
| AGROVOC Term: | Agrobacterium tumefaciens |
| AGROVOC Term: | Hygromycin |
| AGROVOC Term: | PCR |
| AGROVOC Term: | Hybridization |
| AGROVOC Term: | Cultivation |
| AGROVOC Term: | Gene transfer |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12320 |
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