Citation
Zainal Abidin M. A., . and V. Ganesan, . (1999) Cryopreservation of in vitro-produced basidiospores of Oncobasidium theobromae Talbot Keane. [Proceedings Paper]
Abstract
Oncobasidium theobromae Talbot Keane is the causal pathogen of vascular-streak dieback VSD of cocoa in Asia and Papua New Guinea. Disease resistance is considered the most effective for controlling VSD. Resistance screening require a regular supply of basidiospores the only known propagules. A method was developed to produce basidiospores in vitro by exposing cultures to saturated moist air generated by a humidifier. The spores were viable and infective on cocoa. Since they readily germinate in water the ability to preserve them in liquid nitrogen is useful for synchronising the presence of both susceptible host and infective inocula. This paper reports a study to test the viability of in vitro-produced basidiospores of O. theobromae stored in liquid nitrogen.
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Abstract
Oncobasidium theobromae Talbot Keane is the causal pathogen of vascular-streak dieback VSD of cocoa in Asia and Papua New Guinea. Disease resistance is considered the most effective for controlling VSD. Resistance screening require a regular supply of basidiospores the only known propagules. A method was developed to produce basidiospores in vitro by exposing cultures to saturated moist air generated by a humidifier. The spores were viable and infective on cocoa. Since they readily germinate in water the ability to preserve them in liquid nitrogen is useful for synchronising the presence of both susceptible host and infective inocula. This paper reports a study to test the viability of in vitro-produced basidiospores of O. theobromae stored in liquid nitrogen.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. Call number mal SB 950 I61 1999 |
| AGROVOC Term: | Cryopreservation |
| AGROVOC Term: | In vitro |
| AGROVOC Term: | Basidiospores |
| AGROVOC Term: | Oncobasidium theobromae |
| AGROVOC Term: | Pathogens |
| AGROVOC Term: | Disease control |
| AGROVOC Term: | Dieback |
| AGROVOC Term: | Cocoa plant |
| AGROVOC Term: | Disease resistance |
| AGROVOC Term: | Basidiospores |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12332 |
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