Citation
Sariah M., . and Wong M. Y., . and Radzali M., . and Maheran A. A., . (1999) Strain improvement of Trichoderma harzianum Rifai through protoplast fusion. [Proceedings Paper]
Abstract
Three indigenous isolates of Trichoderma harzianum from the rhizospheres of groundnut lMl 378843 chilli IMl378844 and oil palm IMI 37884 1 were used in the protoplast fusion studies. Chemically induced fusion using polyethylene glycol pEG among the three isolates yielded a total of 12 fusants. The fused protoplasts germinated 18h after incubation in liquid Protoplast Regeneration Medium PRM. When plated on agar PRM the fusants regenerated into single colonies between 24-48h after incubation. Of the 12 fusants obtained five fusants showed non-parental type in isozyme analysis. They were further evaluated based on cultural and morphological analysis biomass growth antagonistic activities against Sclerotium rolfsii and Ganoderma boninense and tolerance to three commonly used fungicides. Despite isozyme banding patterns of the five fusants showing non-parental type there was similarity in colony and microscopic appearance with the parental isolates. In the dual culture test fusants D and E showed significantly better antagonism pO.O 1 against both S. rolfsii and G. boninense than both the parental isolates. All the five fusants showed no improvement in biomass growth and tolerance to sublethal doses of Quintozene Propiconazole and Penconazole. However these fusants and their parental isolates showed significantly pO.01 higher tolerance to these fungicides than the target pathogens. Regardless of its genetic basis the diversity of progeny obtained through protoplast fusion in T. harzianum can be used as a source of improved strains for biological control.
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Abstract
Three indigenous isolates of Trichoderma harzianum from the rhizospheres of groundnut lMl 378843 chilli IMl378844 and oil palm IMI 37884 1 were used in the protoplast fusion studies. Chemically induced fusion using polyethylene glycol pEG among the three isolates yielded a total of 12 fusants. The fused protoplasts germinated 18h after incubation in liquid Protoplast Regeneration Medium PRM. When plated on agar PRM the fusants regenerated into single colonies between 24-48h after incubation. Of the 12 fusants obtained five fusants showed non-parental type in isozyme analysis. They were further evaluated based on cultural and morphological analysis biomass growth antagonistic activities against Sclerotium rolfsii and Ganoderma boninense and tolerance to three commonly used fungicides. Despite isozyme banding patterns of the five fusants showing non-parental type there was similarity in colony and microscopic appearance with the parental isolates. In the dual culture test fusants D and E showed significantly better antagonism pO.O 1 against both S. rolfsii and G. boninense than both the parental isolates. All the five fusants showed no improvement in biomass growth and tolerance to sublethal doses of Quintozene Propiconazole and Penconazole. However these fusants and their parental isolates showed significantly pO.01 higher tolerance to these fungicides than the target pathogens. Regardless of its genetic basis the diversity of progeny obtained through protoplast fusion in T. harzianum can be used as a source of improved strains for biological control.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. Call number mal SB 950 I61 1999 |
| AGROVOC Term: | Trichoderma harzianum |
| AGROVOC Term: | Protoplasts |
| AGROVOC Term: | Rhizosphere |
| AGROVOC Term: | Groundnuts |
| AGROVOC Term: | Polyethylene |
| AGROVOC Term: | Isozymes |
| AGROVOC Term: | Biomass |
| AGROVOC Term: | morphology |
| AGROVOC Term: | Sclerotium rolfsii |
| AGROVOC Term: | Fungicides |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12333 |
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