Advances in redox proteomics and metabolomics: application to oil palm

Plowman J., . and Thomas A., . and Clerens S., . and Santanu D. C., . and Dyer J., . and Umi S. Ramli, . and Cornellison C., . and Koehn H., . and Grosvenor A., . (2009) Advances in redox proteomics and metabolomics: application to oil palm. [Proceedings Paper]

The science of proteomics is less advanced in plants than equivalent studies in prokaryotes simple eukaryotes and several mammalian systems. Many aspects of plant proteomics can benefit from more intensive research in proteomics to the solution of practical problems in metabolic pathways plants response to disease and stress conditions which will give an added dimension to the already recognized importance of research in plant proteomics. The application of proteomic approaches to plant entails comprehensive identification of proteins characterization and function of each protein and analysis of protein regulation. Oil palm is an important commodity crop supplying a major portion of the world;s vegetable oil. Sequencing ofthe oil palm genome is in its final stage and thus proteomics would be a powerful tool for its functional analysis. Recent developments in oil palm proteomics have been enabled through an integrated proteomics platform which involved the use of shotgun approach such as tandem mass spectrometry combined with 2-D gel electrophoresis and reverse phase chromatography many of which dealing with integrating the technologies to the transcriptomics towards novel products development and understanding naturally occurring disease states as an alternative discovery-based approach for biomarker discovery. The emerging disciplines of redox proteomics and metabolomics encompass the utilisation of powerful mass spectrometric tools to characterise and understand key oxidation and reduction events within proteins and other metabolites. These tools are therefore ideally suited for studying damage caused through the generation and attack of reactive oxygen speciesROS. We have developed and applied advanced protocols to identify profile locate and track oxidative modification in a range of proteins lipids and secondary metabolites including keratins collagens fatty acids stilbenes and coumarins. The development and members. Composting systems and usage of compost for substitution of inorganic fertilizer and its impact on yield enhancement are described in the paper. The 4 Rs approach to fertilizer management Roberts 2007 are also discussed in relation to compost usage. The oil palm industry is strongly urged to adopt the good agricultural practice in the establishment of leguminous cover crops for N fixation without fail in all new plantings/replantings. The choice of Mucuna bracteata over other conventional leguminous cover crops is strongly emphasized for reasons discussed. The potential usage of biochar derivable from the oil palm biomass is also highlighted as an area of research that can lead to its future exploitation for soil fertility improvement and long term sequestration of CO2 in soil.

Full text available from:

Abstract

The science of proteomics is less advanced in plants than equivalent studies in prokaryotes simple eukaryotes and several mammalian systems. Many aspects of plant proteomics can benefit from more intensive research in proteomics to the solution of practical problems in metabolic pathways plants response to disease and stress conditions which will give an added dimension to the already recognized importance of research in plant proteomics. The application of proteomic approaches to plant entails comprehensive identification of proteins characterization and function of each protein and analysis of protein regulation. Oil palm is an important commodity crop supplying a major portion of the world;s vegetable oil. Sequencing ofthe oil palm genome is in its final stage and thus proteomics would be a powerful tool for its functional analysis. Recent developments in oil palm proteomics have been enabled through an integrated proteomics platform which involved the use of shotgun approach such as tandem mass spectrometry combined with 2-D gel electrophoresis and reverse phase chromatography many of which dealing with integrating the technologies to the transcriptomics towards novel products development and understanding naturally occurring disease states as an alternative discovery-based approach for biomarker discovery. The emerging disciplines of redox proteomics and metabolomics encompass the utilisation of powerful mass spectrometric tools to characterise and understand key oxidation and reduction events within proteins and other metabolites. These tools are therefore ideally suited for studying damage caused through the generation and attack of reactive oxygen speciesROS. We have developed and applied advanced protocols to identify profile locate and track oxidative modification in a range of proteins lipids and secondary metabolites including keratins collagens fatty acids stilbenes and coumarins. The development and members. Composting systems and usage of compost for substitution of inorganic fertilizer and its impact on yield enhancement are described in the paper. The 4 Rs approach to fertilizer management Roberts 2007 are also discussed in relation to compost usage. The oil palm industry is strongly urged to adopt the good agricultural practice in the establishment of leguminous cover crops for N fixation without fail in all new plantings/replantings. The choice of Mucuna bracteata over other conventional leguminous cover crops is strongly emphasized for reasons discussed. The potential usage of biochar derivable from the oil palm biomass is also highlighted as an area of research that can lead to its future exploitation for soil fertility improvement and long term sequestration of CO2 in soil.

[error in script]

Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Darul Ehsan Malaysia. Call Number mal TP684 P3I61 2009 vol. 1
AGROVOC Term:	Oil palm
AGROVOC Term:	prokaryotes
AGROVOC Term:	Plant protein
AGROVOC Term:	Genomes
AGROVOC Term:	Mass spectrometry
AGROVOC Term:	Electrophoresis
AGROVOC Term:	Biomarkers of exposure organisms
AGROVOC Term:	Oxidation
AGROVOC Term:	reactive oxygen species
AGROVOC Term:	Metabolites
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/12367

Actions (login required)

View Item