Citation
Tengku Laila Kamaliah, . and Azmi A. Rashid, . and Maheran A. Aziz, . and Siti Aishah Hassan, . (2001) Effects of cytokinin on multiple shoot formation from shoot tips of cabbage Brassica oleracea subsp. capitata cv. tropicana. [Proceedings Paper]
Abstract
Conditions for plant regeneration from excised shoot tips of Brassica oleracea subsp. capitata were studied. The aim of this study was to determine suitable concentration of cytokinin for multiple shoot formation. Seven days old shoot tips were excised from in vitro-grown seedlings and cultured on MS medium containing N6-benzyladenine BA at 3.6 9 12 15 and 18 mg/l 13.3 26.6 39.9 53.2 66.5 and 79.8 M or 6-furfuryl aminopurine kinetin at 6 12 18 24 30 and 36 mg/l 27.8 55.6 83.4 111.2. 139 and 166.8 M. The addition of cytokinin induced a variable amount of callus at the base of the shoot tip followed by multiple shoot formation. The highest mean number of transferable shoots per explant and the highest percentage of shoot formation were produced on 3 and 6 mg BA per liter 13.3 and 26.6 M respectively. On kinetin-containing media the highest mean number of transferable shoots per explant and the highest percentage of shoot formation were attained on 6 mg/l kinetin. Multiple shoots were formed within 4 weeks. Regenerated shoots were rooted on hormone-free MS medium.
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Abstract
Conditions for plant regeneration from excised shoot tips of Brassica oleracea subsp. capitata were studied. The aim of this study was to determine suitable concentration of cytokinin for multiple shoot formation. Seven days old shoot tips were excised from in vitro-grown seedlings and cultured on MS medium containing N6-benzyladenine BA at 3.6 9 12 15 and 18 mg/l 13.3 26.6 39.9 53.2 66.5 and 79.8 M or 6-furfuryl aminopurine kinetin at 6 12 18 24 30 and 36 mg/l 27.8 55.6 83.4 111.2. 139 and 166.8 M. The addition of cytokinin induced a variable amount of callus at the base of the shoot tip followed by multiple shoot formation. The highest mean number of transferable shoots per explant and the highest percentage of shoot formation were produced on 3 and 6 mg BA per liter 13.3 and 26.6 M respectively. On kinetin-containing media the highest mean number of transferable shoots per explant and the highest percentage of shoot formation were attained on 6 mg/l kinetin. Multiple shoots were formed within 4 weeks. Regenerated shoots were rooted on hormone-free MS medium.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK 710.2 M239 2001 Call Number |
| AGROVOC Term: | Brassica oleracea capitata |
| AGROVOC Term: | Brassicaceae |
| AGROVOC Term: | Cabbages |
| AGROVOC Term: | Cytokinins |
| AGROVOC Term: | Seeds |
| AGROVOC Term: | Culture media |
| AGROVOC Term: | Benzyladenine |
| AGROVOC Term: | Kinetin |
| AGROVOC Term: | In vitro culture |
| AGROVOC Term: | Culture techniques |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12483 |
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