Citation
Lim S. P., . and Uma R. S., . (2001) Towards the development of a protocol for cryopreservation of petai Parkja speciosa hassk. zygotic embryos. [Proceedings Paper]
Abstract
This study was carried out to evaluate the potential of cryopreservation technique for germplasm conservation of petai zygotic embryos. The effect of various desiccation periods and different concentration of sucrose pretreatment on survival of petai embryos in liquid nitrogen LN was evaluated. The result shows that non-pretreated petai embryos were sensitive to desiccation whereby a reduction of moisture content to below 16 decreased survival percentage to less than 48. On freezing no embryos survived irrespective of their moisture content. Sucrose pretreatment was found to enhance desiccation and freezing tolerance in petai embryos whereby survival percentage of approximately 90 and 50 was observed without and with LN exposure respectively.
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Abstract
This study was carried out to evaluate the potential of cryopreservation technique for germplasm conservation of petai zygotic embryos. The effect of various desiccation periods and different concentration of sucrose pretreatment on survival of petai embryos in liquid nitrogen LN was evaluated. The result shows that non-pretreated petai embryos were sensitive to desiccation whereby a reduction of moisture content to below 16 decreased survival percentage to less than 48. On freezing no embryos survived irrespective of their moisture content. Sucrose pretreatment was found to enhance desiccation and freezing tolerance in petai embryos whereby survival percentage of approximately 90 and 50 was observed without and with LN exposure respectively.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK 710.2 M239 2001 Call Number |
| AGROVOC Term: | Parkia |
| AGROVOC Term: | Fabaceae |
| AGROVOC Term: | Cryopreservation |
| AGROVOC Term: | Germplasm conservation |
| AGROVOC Term: | Desiccation physiology |
| AGROVOC Term: | Seed |
| AGROVOC Term: | Culture media |
| AGROVOC Term: | Charcoal |
| AGROVOC Term: | Naphthaleneacetic acid |
| AGROVOC Term: | Benzyladenine |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12488 |
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