Citation
Rosmin Kasran, . and Hafezah Mohd, . and Zainal Abidin Mior Ahmad, . and Radzali Muse, . and Nurhayati Yusoff, . and Johari Ramli, . (2001) Biochemical and molecular studies on the interaction between Theobroma cacao L. and their fungal pathogens in vitro. [Proceedings Paper]
Abstract
In Malaysia Theobroma cacao L. cocoa plants of Sterculiasae family are commonly dealt with several endemic diseases such as vascular streak diebackVSD and black pod BP diseases causing by the fungal Oncobasidium theobromae TALBOT and KEANE and Phytophthora palmivora respectively. Biotechnology and molecular biology studies were used to investigate the biochemical and molecular biology markers of resistant and susceptible cocoa clones. Crude culture filtrates of the particular fungal pathogen were bioassayed in vitro with cocoa leaf disks callus portions and cell-suspension cultures. The resistant clones were found highly produced polyphenols compared with the susceptible clones. When dual-culture technigue an isolated fungal pathogen O. theobromae grew together with the cocoa tissues was applied the tissue resistant clonesICS95 KKM25 resulting in less hyphae compared to those susceptible clones NA32 PA 7. However electron miscroscopicEM studies showed that the infected tissues of susceptible cocoa clones showed some damage symptoms such cell shrinkage and plasmolysed cells compared to less in the resistant clones. The finding of AFLP technique was markedly able to differentiate between cocoa clones that are resistant and susceptible to a VSD disease using selected primer combinations and this showed polymorphism among the cocoa clones.
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Abstract
In Malaysia Theobroma cacao L. cocoa plants of Sterculiasae family are commonly dealt with several endemic diseases such as vascular streak diebackVSD and black pod BP diseases causing by the fungal Oncobasidium theobromae TALBOT and KEANE and Phytophthora palmivora respectively. Biotechnology and molecular biology studies were used to investigate the biochemical and molecular biology markers of resistant and susceptible cocoa clones. Crude culture filtrates of the particular fungal pathogen were bioassayed in vitro with cocoa leaf disks callus portions and cell-suspension cultures. The resistant clones were found highly produced polyphenols compared with the susceptible clones. When dual-culture technigue an isolated fungal pathogen O. theobromae grew together with the cocoa tissues was applied the tissue resistant clonesICS95 KKM25 resulting in less hyphae compared to those susceptible clones NA32 PA 7. However electron miscroscopicEM studies showed that the infected tissues of susceptible cocoa clones showed some damage symptoms such cell shrinkage and plasmolysed cells compared to less in the resistant clones. The finding of AFLP technique was markedly able to differentiate between cocoa clones that are resistant and susceptible to a VSD disease using selected primer combinations and this showed polymorphism among the cocoa clones.
Additional Metadata
Item Type: | Proceedings Paper |
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Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK 710.2 M239 2001 Call Number |
AGROVOC Term: | Theobroma cacao |
AGROVOC Term: | Sterculiaceae |
AGROVOC Term: | Cocoa plant |
AGROVOC Term: | Clones |
AGROVOC Term: | Fungal diseases |
AGROVOC Term: | Molecular biology |
AGROVOC Term: | Oncobasidium theobromae |
AGROVOC Term: | Phytophthora palmivora |
AGROVOC Term: | In vitro |
AGROVOC Term: | Plant diseases |
Geographical Term: | MALAYSIA |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 24 Apr 2025 05:15 |
URI: | http://webagris.upm.edu.my/id/eprint/12493 |
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