Biochemical and molecular studies on the interaction between Theobroma cacao L. and their fungal pathogens in vitro

Rosmin Kasran, . and Hafezah Mohd, . and Zainal Abidin Mior Ahmad, . and Radzali Muse, . and Nurhayati Yusoff, . and Johari Ramli, . (2001) Biochemical and molecular studies on the interaction between Theobroma cacao L. and their fungal pathogens in vitro. [Proceedings Paper]

In Malaysia Theobroma cacao L. cocoa plants of Sterculiasae family are commonly dealt with several endemic diseases such as vascular streak diebackVSD and black pod BP diseases causing by the fungal Oncobasidium theobromae TALBOT and KEANE and Phytophthora palmivora respectively. Biotechnology and molecular biology studies were used to investigate the biochemical and molecular biology markers of resistant and susceptible cocoa clones. Crude culture filtrates of the particular fungal pathogen were bioassayed in vitro with cocoa leaf disks callus portions and cell-suspension cultures. The resistant clones were found highly produced polyphenols compared with the susceptible clones. When dual-culture technigue an isolated fungal pathogen O. theobromae grew together with the cocoa tissues was applied the tissue resistant clonesICS95 KKM25 resulting in less hyphae compared to those susceptible clones NA32 PA 7. However electron miscroscopicEM studies showed that the infected tissues of susceptible cocoa clones showed some damage symptoms such cell shrinkage and plasmolysed cells compared to less in the resistant clones. The finding of AFLP technique was markedly able to differentiate between cocoa clones that are resistant and susceptible to a VSD disease using selected primer combinations and this showed polymorphism among the cocoa clones.

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Abstract

In Malaysia Theobroma cacao L. cocoa plants of Sterculiasae family are commonly dealt with several endemic diseases such as vascular streak diebackVSD and black pod BP diseases causing by the fungal Oncobasidium theobromae TALBOT and KEANE and Phytophthora palmivora respectively. Biotechnology and molecular biology studies were used to investigate the biochemical and molecular biology markers of resistant and susceptible cocoa clones. Crude culture filtrates of the particular fungal pathogen were bioassayed in vitro with cocoa leaf disks callus portions and cell-suspension cultures. The resistant clones were found highly produced polyphenols compared with the susceptible clones. When dual-culture technigue an isolated fungal pathogen O. theobromae grew together with the cocoa tissues was applied the tissue resistant clonesICS95 KKM25 resulting in less hyphae compared to those susceptible clones NA32 PA 7. However electron miscroscopicEM studies showed that the infected tissues of susceptible cocoa clones showed some damage symptoms such cell shrinkage and plasmolysed cells compared to less in the resistant clones. The finding of AFLP technique was markedly able to differentiate between cocoa clones that are resistant and susceptible to a VSD disease using selected primer combinations and this showed polymorphism among the cocoa clones.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK 710.2 M239 2001 Call Number
AGROVOC Term:	Theobroma cacao
AGROVOC Term:	Sterculiaceae
AGROVOC Term:	Cocoa plant
AGROVOC Term:	Clones
AGROVOC Term:	Fungal diseases
AGROVOC Term:	Molecular biology
AGROVOC Term:	Oncobasidium theobromae
AGROVOC Term:	Phytophthora palmivora
AGROVOC Term:	In vitro
AGROVOC Term:	Plant diseases
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/12493

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