Identification of different oil palm clones using microsatellite markers


Citation

Praveena Tangayat, . and Aziah Mohamad Yusoff, . and Mohaimi Mohamad, . and Khairudin Hashim, . and Bakoume C. C., . and Harikrishna Kulaveerasingam, . and Jangi Mohamad Sanusi, . and Suzaini Yahya, . (2009) Identification of different oil palm clones using microsatellite markers. [Proceedings Paper]

Abstract

Since oil palm Elaeis guineensis Jacq. does not breed true tissue culture-derived material is desirable for homogeneity of oil palms vis- -vis yield and growth traits as well as resistance to diseases. However sources of clonal material for oil palm growers vary in yield expectations and selling prices offered by tissue culturists hence the necessity to seek ways of identifying clones particularly in the case of unsatisfactory performance of materials usually planted in bulk. Oil palm shoots from five different clones were screened with ten microsatellite markers. The genetic diversity observed was high He 0.605 perhaps due to intrinsic heterozygosity of oil palm in its out-crossing behaviour. The mean genetic distance among clones was average 0.506 ranging from 0.050 to 0.703. A cluster analysis using the Unweighted Pair Group Method with Arithmetic UPGMA clustering method based on Nei;s 1983 genetic distance showed five different sub-clusters each grouping shoots from the same ortet. The five subclusters correspond to the five clones. These findings demonstrate that these ten microsatellite markers are potential tools for clone identification.


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Abstract

Since oil palm Elaeis guineensis Jacq. does not breed true tissue culture-derived material is desirable for homogeneity of oil palms vis- -vis yield and growth traits as well as resistance to diseases. However sources of clonal material for oil palm growers vary in yield expectations and selling prices offered by tissue culturists hence the necessity to seek ways of identifying clones particularly in the case of unsatisfactory performance of materials usually planted in bulk. Oil palm shoots from five different clones were screened with ten microsatellite markers. The genetic diversity observed was high He 0.605 perhaps due to intrinsic heterozygosity of oil palm in its out-crossing behaviour. The mean genetic distance among clones was average 0.506 ranging from 0.050 to 0.703. A cluster analysis using the Unweighted Pair Group Method with Arithmetic UPGMA clustering method based on Nei;s 1983 genetic distance showed five different sub-clusters each grouping shoots from the same ortet. The five subclusters correspond to the five clones. These findings demonstrate that these ten microsatellite markers are potential tools for clone identification.

Additional Metadata

[error in script]
Item Type: Proceedings Paper
Additional Information: Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP 684 P3 161 2009 vol. 2 Call Number.
AGROVOC Term: Elaeis guineensis
AGROVOC Term: Oil palms
AGROVOC Term: Microsatellites
AGROVOC Term: Genetic markers
AGROVOC Term: Identification
AGROVOC Term: Shoots
AGROVOC Term: Extraction
AGROVOC Term: Purification
AGROVOC Term: Loci
AGROVOC Term: DNA
Geographical Term: MALAYSIA
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 05:15
URI: http://webagris.upm.edu.my/id/eprint/12553

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