Citation
Chan K. L., . and Leslie L. E. T., . and Singh R., . (2009) Identification and localization of simple sequence repeats for genes encoding fatty acid biosynthetic enzymes. [Proceedings Paper]
Abstract
Palm oil is one of the world;s most widely consumed edible oils. The oil contains about 44 palmitic acid CI6:0 39 oleic acid CI8:I 10 linoleic acid CI8:2 and less than 5 stearic acid CI8:0. The fatty acid composition varies and can be attributed to the interactions between several genes. Mapping the underlying genes or quantitative trait locus QTLs controlling fatty acid composition would allow the application of marker-assisted selection in oil palm breeding. In this effort genes associated with fatty acid biosynthesis such as beta-ketoacyl-ACP synthase II palmitoyl-ACP thioesterase and stearoyl-ACP desaturase were identified from the sequence collection. Simple sequence repeats SSRswere identified and localized using MISA the MIcroSAtellite identification tool. In this study SSRs were found in different regions of the putative genes. For example mononucleotide repeats were identified in enoyl-ACP reductase palmitoyl-ACP thioesterase and oleoyl-ACP thioesterase while tetranucleotide repeats were identified in KAS II. SSRs are valuable as genetic markers because they are co-dominant detect high levels of allelic diversity and are easily and economically assayed by the polymerase chain reaction PCR. PCR primers were designed flanking the SSR locus using Primed3 a widely used program for designing PCR primers. The SSRs are being mapped into the oil palm mapping populations. SSRs have proved to be versatile molecular markers particularly for genome mapping and other population analysis.
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Abstract
Palm oil is one of the world;s most widely consumed edible oils. The oil contains about 44 palmitic acid CI6:0 39 oleic acid CI8:I 10 linoleic acid CI8:2 and less than 5 stearic acid CI8:0. The fatty acid composition varies and can be attributed to the interactions between several genes. Mapping the underlying genes or quantitative trait locus QTLs controlling fatty acid composition would allow the application of marker-assisted selection in oil palm breeding. In this effort genes associated with fatty acid biosynthesis such as beta-ketoacyl-ACP synthase II palmitoyl-ACP thioesterase and stearoyl-ACP desaturase were identified from the sequence collection. Simple sequence repeats SSRswere identified and localized using MISA the MIcroSAtellite identification tool. In this study SSRs were found in different regions of the putative genes. For example mononucleotide repeats were identified in enoyl-ACP reductase palmitoyl-ACP thioesterase and oleoyl-ACP thioesterase while tetranucleotide repeats were identified in KAS II. SSRs are valuable as genetic markers because they are co-dominant detect high levels of allelic diversity and are easily and economically assayed by the polymerase chain reaction PCR. PCR primers were designed flanking the SSR locus using Primed3 a widely used program for designing PCR primers. The SSRs are being mapped into the oil palm mapping populations. SSRs have proved to be versatile molecular markers particularly for genome mapping and other population analysis.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP 684 P3 161 2009 vol. 2 Call Number. |
| AGROVOC Term: | Oil palms |
| AGROVOC Term: | Fatty acids |
| AGROVOC Term: | Genes |
| AGROVOC Term: | Genetic markers |
| AGROVOC Term: | Biosynthesis |
| AGROVOC Term: | Identification |
| AGROVOC Term: | Enzymes |
| AGROVOC Term: | Microsatellites |
| AGROVOC Term: | Polymorphism |
| AGROVOC Term: | QTL quantitative trait loci |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12559 |
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