Citation
Asenjo B. G., . and Croxford A. E., . and Wilkinson M. J., . and Nelson S. P. C., . and Fillianti H., . and Caligari P. D. S., . and Forster B. P., . (2009) Single nucleotide polymorphism SNP detection using high resolution melt PCR. [Proceedings Paper]
Abstract
High resolution melting HRM polymerase chain reaction PCR of DNA has been developed as an advanced technology in detecting single nucleotide polymorphisms SNPs. The technique exploits real time PCR differential DNA melting characteristics caused by sequence polymorphism and has advantages of being a non-gel system. The technology requires no sequence information and has the potential to be automated. HRM PCR detects mutations in DNA and differentiates homozygous and heterozygous genotypes through their different DNA melting curves. The method is used routinely at Sumatra Bioscience in detecting polymorphism at specific loci in the oil palm Elaeis guineensis Jacq. genome. Sequence analysis of DNA showing different melt curves has revealed exchanges in single bases e.g. A -T with G-C or vice versa. The detected SNPs can be further exploited in developing an allelic series for the locus examined and in developing haplotypes.
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Abstract
High resolution melting HRM polymerase chain reaction PCR of DNA has been developed as an advanced technology in detecting single nucleotide polymorphisms SNPs. The technique exploits real time PCR differential DNA melting characteristics caused by sequence polymorphism and has advantages of being a non-gel system. The technology requires no sequence information and has the potential to be automated. HRM PCR detects mutations in DNA and differentiates homozygous and heterozygous genotypes through their different DNA melting curves. The method is used routinely at Sumatra Bioscience in detecting polymorphism at specific loci in the oil palm Elaeis guineensis Jacq. genome. Sequence analysis of DNA showing different melt curves has revealed exchanges in single bases e.g. A -T with G-C or vice versa. The detected SNPs can be further exploited in developing an allelic series for the locus examined and in developing haplotypes.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP 684 P3 161 2009 vol. 2 Call Number. |
| AGROVOC Term: | Elaeis guineensis |
| AGROVOC Term: | Oil palms |
| AGROVOC Term: | Techniques |
| AGROVOC Term: | Polymerase chain reaction |
| AGROVOC Term: | Genetics |
| AGROVOC Term: | Molecular genetics |
| AGROVOC Term: | Leaves |
| AGROVOC Term: | DNA |
| AGROVOC Term: | Germplasm |
| AGROVOC Term: | Genotypes |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12570 |
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