Citation
Reflini, . and Forster B. P., . and Nelson S. P. C., . and Caligari P. D. S., . (2009) Factors influencing callogenesis and embryogenesis in oil palm cultures. [Proceedings Paper]
Abstract
A number of factors influence tissue culture of oil palm Elaeis guineensis Jacq. for clone production. These include: composition of the culture medium culture environment and genotype of the source material. The concentration combination and sequence of growth regulators are key factors for tissue culture success but carbohydrate source has also been identified as having a major influence on callus and embryo induction. Maltose was found to be the best carbohydrate source for embryogenesis. MS culture medium that contained 3 maltose as a carbohydrate source was found to be better than media containing 3 sucrose. Increased sucrose concentrations up to 6 decreased callus and somatic embryo production. Of the three sugars tested glucose gave the poorest results in terms of callus and embryo production. In further experiments with auxins at the pretreatment liquid medium stage it was found that all treatments of 7.5 ppm of 2 4-D rather than 5 ppm resulted in more embryos at subsequent culture stages.
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Abstract
A number of factors influence tissue culture of oil palm Elaeis guineensis Jacq. for clone production. These include: composition of the culture medium culture environment and genotype of the source material. The concentration combination and sequence of growth regulators are key factors for tissue culture success but carbohydrate source has also been identified as having a major influence on callus and embryo induction. Maltose was found to be the best carbohydrate source for embryogenesis. MS culture medium that contained 3 maltose as a carbohydrate source was found to be better than media containing 3 sucrose. Increased sucrose concentrations up to 6 decreased callus and somatic embryo production. Of the three sugars tested glucose gave the poorest results in terms of callus and embryo production. In further experiments with auxins at the pretreatment liquid medium stage it was found that all treatments of 7.5 ppm of 2 4-D rather than 5 ppm resulted in more embryos at subsequent culture stages.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP 684 P3 161 2009 vol. 2 Call Number. |
| AGROVOC Term: | Elaeis guineensis |
| AGROVOC Term: | Oil palms |
| AGROVOC Term: | Callogenesis |
| AGROVOC Term: | Embryogenesis |
| AGROVOC Term: | Micropropagation |
| AGROVOC Term: | Culture media |
| AGROVOC Term: | Genotypes |
| AGROVOC Term: | Explants |
| AGROVOC Term: | Leaves |
| AGROVOC Term: | Callus |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12571 |
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