Citation
Forster B. P., . and Rusfiandi H., . and Nelson S. P. C., . and Nasution O., . and Sitorus A. C., . and Caligari P. D. S., . (2009) Cytological studies of pollen development in oil palm Elaeis guineensis Jacq. [Proceedings Paper]
Abstract
Pollen the mature male gametophyte of plants plays an important role in the reproductive cycle of higher plants. The development of oil palm pollen was studied cytologically in all stages: from the pollen mother cell to mature pollen. Mature pollen was germinated in vitro to investigate features of the vegetative and generative nuclei development inside the pollen tube. Meiosis of the oil palm pollen mother cell results in a tetrad of four microspores. At separation from the pyramidal tetrad each microspore is uni-nucleate and appears to be empty deformed and lacking in cytoplasm. The microspore then develops into a well-defined structure with dense cytoplasm. Starch is accumulated at later stages. Asymmetrical mitosis of the microspore produces a large vegetative cell and a smaller generative cell. This results in a bi-nucleate pollen grain. The behaviour of the two distinct nuclei during pollen germination in vitro was examined using DAPI staining. Both vegetative and generative nuclei entered the pollen tube at 3h. At 18 h the generative nucleus divided to form two sperm cells. One of the sperm cells became associated with the vegetative nucleus at 20 h.
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Abstract
Pollen the mature male gametophyte of plants plays an important role in the reproductive cycle of higher plants. The development of oil palm pollen was studied cytologically in all stages: from the pollen mother cell to mature pollen. Mature pollen was germinated in vitro to investigate features of the vegetative and generative nuclei development inside the pollen tube. Meiosis of the oil palm pollen mother cell results in a tetrad of four microspores. At separation from the pyramidal tetrad each microspore is uni-nucleate and appears to be empty deformed and lacking in cytoplasm. The microspore then develops into a well-defined structure with dense cytoplasm. Starch is accumulated at later stages. Asymmetrical mitosis of the microspore produces a large vegetative cell and a smaller generative cell. This results in a bi-nucleate pollen grain. The behaviour of the two distinct nuclei during pollen germination in vitro was examined using DAPI staining. Both vegetative and generative nuclei entered the pollen tube at 3h. At 18 h the generative nucleus divided to form two sperm cells. One of the sperm cells became associated with the vegetative nucleus at 20 h.
Additional Metadata
Item Type: | Proceedings Paper |
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Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP 684 P3 161 2009 vol. 2 Call Number. |
AGROVOC Term: | Elaeis guineensis |
AGROVOC Term: | Oil palms |
AGROVOC Term: | Pollen |
AGROVOC Term: | Cytological analysis |
AGROVOC Term: | Cytoplasm |
AGROVOC Term: | Cell division |
AGROVOC Term: | Microspores pollen |
AGROVOC Term: | Pollination |
AGROVOC Term: | Buds |
AGROVOC Term: | In vitro culture |
Geographical Term: | MALAYSIA |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 24 Apr 2025 05:15 |
URI: | http://webagris.upm.edu.my/id/eprint/12574 |
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