Citation
Forster. B. P., . and Setiawati U., . and Nelson S. P. C., . and Nasution O., . and Sitorus A. C., . and Caligari P. D. S., . (2009) Determination of oil palm pollen fertility using staining techniques. [Proceedings Paper]
Abstract
The accurate determination of pollen fertility in oil palm Elaeis guineensis Jacq. is of critical importance in pollen storage as well as in germplasm exchange especially for use in breeding. Pollen can be categorized as non-viable dead viable alive but not necessarily fertile and fertile potent pollen. It is important in practical terms to be able to differentiate between these three categories and to determine their frequencies in a pollen sample. The effectiveness of different staining methods using fluorescein diacetate FDA aceto-orcein 4 ;6-diamidino-2-phenylindole DAP1 and pollen germination to predict pollen fertility were investigated. Although all of these methods gave similar results FDA staining was found to be the best method in terms of time and resource efficiency.
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Abstract
The accurate determination of pollen fertility in oil palm Elaeis guineensis Jacq. is of critical importance in pollen storage as well as in germplasm exchange especially for use in breeding. Pollen can be categorized as non-viable dead viable alive but not necessarily fertile and fertile potent pollen. It is important in practical terms to be able to differentiate between these three categories and to determine their frequencies in a pollen sample. The effectiveness of different staining methods using fluorescein diacetate FDA aceto-orcein 4 ;6-diamidino-2-phenylindole DAP1 and pollen germination to predict pollen fertility were investigated. Although all of these methods gave similar results FDA staining was found to be the best method in terms of time and resource efficiency.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP 684 P3 161 2009 vol. 2 Call Number. |
| AGROVOC Term: | Elaeis guineensis |
| AGROVOC Term: | Oil palms |
| AGROVOC Term: | Pollen |
| AGROVOC Term: | Fertility |
| AGROVOC Term: | Crop production |
| AGROVOC Term: | Plant developmental stages |
| AGROVOC Term: | In vitro |
| AGROVOC Term: | Sucrose |
| AGROVOC Term: | Plant breeding |
| AGROVOC Term: | Pollen germination |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12576 |
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