Citation
Yong W., . and Janna Ong Abdullah, . and Maziah M., . (2004) Parameters optimization of Agrobacterium-mediated transformation of Melastoma malabathricum. [Proceedings Paper]
Abstract
Transformation of Melastoma malabathricum with green fluorescent protein GFP and -glucuronidase GUS genes using Agrobacterium-mediated method was studied. The effects of immersion time types of wounding acetosyringone and Agrobacterium concentration known to influence transformation efficiency were assessed. Results were based on the percentage of GFP expression which was observed three days after transformation. Histochemical GUS analysis confirmed that the reporter gene was transferred to the cells and was successfully expressed. In the presence of hygromycin putative transformants containing hygromycinresistant gene survived. The indentation sites of leaves were found to be the major sites of infection. Callus formed at the edges of leaves also showed GFP expression.
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Abstract
Transformation of Melastoma malabathricum with green fluorescent protein GFP and -glucuronidase GUS genes using Agrobacterium-mediated method was studied. The effects of immersion time types of wounding acetosyringone and Agrobacterium concentration known to influence transformation efficiency were assessed. Results were based on the percentage of GFP expression which was observed three days after transformation. Histochemical GUS analysis confirmed that the reporter gene was transferred to the cells and was successfully expressed. In the presence of hygromycin putative transformants containing hygromycinresistant gene survived. The indentation sites of leaves were found to be the major sites of infection. Callus formed at the edges of leaves also showed GFP expression.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2004 Call Number |
| AGROVOC Term: | Melastomataceae |
| AGROVOC Term: | Flowers |
| AGROVOC Term: | Agrobacterium |
| AGROVOC Term: | Genetic transformation |
| AGROVOC Term: | In vitro plantlets |
| AGROVOC Term: | Plasmids |
| AGROVOC Term: | Kanamycin |
| AGROVOC Term: | Hygromycin |
| AGROVOC Term: | Callus culture |
| AGROVOC Term: | Genetic parameters |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12745 |
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