Citation
Lau H. Y., . and Habibuddin H., . and Zamri I., . and Tan C. S., . (2008) Cloning and sequencing of a full length outer membrane protein omp gene of Candidatus liberibacter asiaticus causing citrus greening disease. [Proceedings Paper]
Abstract
Citrus Greening is one of the most serious diseases affecting citrus cultivation in many countries including Malaysia. The disease is caused by a non-cultured phloem-restricted bacterium-like organism Candidatus Liberibacter asiaticus. Its uncliiturable and the low concentration in the infected plants make it difficult to obtain sufficient amount of the pathogen to be used as antigen in the production of its antibodies. Recombinant protein of its outer membrane protein omp can be used as the antigen to produce the antibodies against this Greening disease. In this study the gene encoding the omp of the organism was amplified using the total DNA extracted from disease infected citrus midribs. The amplified PCR fragment of the gene 2.3 kb was then purified restriction enzyme digested and ligated into a bacterial expression plasmid vector pRSET B. The construct was verified by restriction enzyme and DNA sequencing. This full length gene of omp will be used in our subsequent studies to obtain the recombinant protein.
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Abstract
Citrus Greening is one of the most serious diseases affecting citrus cultivation in many countries including Malaysia. The disease is caused by a non-cultured phloem-restricted bacterium-like organism Candidatus Liberibacter asiaticus. Its uncliiturable and the low concentration in the infected plants make it difficult to obtain sufficient amount of the pathogen to be used as antigen in the production of its antibodies. Recombinant protein of its outer membrane protein omp can be used as the antigen to produce the antibodies against this Greening disease. In this study the gene encoding the omp of the organism was amplified using the total DNA extracted from disease infected citrus midribs. The amplified PCR fragment of the gene 2.3 kb was then purified restriction enzyme digested and ligated into a bacterial expression plasmid vector pRSET B. The construct was verified by restriction enzyme and DNA sequencing. This full length gene of omp will be used in our subsequent studies to obtain the recombinant protein.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2008 Call Number |
| AGROVOC Term: | Citrus |
| AGROVOC Term: | Greening |
| AGROVOC Term: | Cultivation |
| AGROVOC Term: | Phloem |
| AGROVOC Term: | Infection |
| AGROVOC Term: | Antibodies |
| AGROVOC Term: | Extraction |
| AGROVOC Term: | Plasmids |
| AGROVOC Term: | DNA sequence |
| AGROVOC Term: | Recombinant proteins |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12771 |
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