Citation
Zuraida A. R., . and Naziah B., . and Nur Farahiyah M. P., . (2008) Stages of seed on callus induction of malaysia indica rice MR219. [Proceedings Paper]
Abstract
Establishment of a highly efficient and widely used tissue culture system for indica rice will accelerate the application of transformation technology. A frequency of obtained callus from seed depends highly on the geno type and stages of seeds. Eight stages of rice seeds variety MR 219 were tested for their callus induction response. By manipulating stages of seed the experiment was conducted to observe their effect on callus response. The stages were: immature/milky half mature mature fresh harvested or 1-6 months after harvest. The callus induction was on the MS basal medium with 30g/L sucrose 3.2g/L phytagel and 1 mg/L 2 4-D in combination with 10 mg/L NAA. Callus responses were obtained for each of the tested stages with a maximum at half mature stage 2.5g callus/plate. The used of seed for callus induction at five months-old and above are not recommended due to the reduction infresh weight of callus.
Download File
Full text available from:
|
Abstract
Establishment of a highly efficient and widely used tissue culture system for indica rice will accelerate the application of transformation technology. A frequency of obtained callus from seed depends highly on the geno type and stages of seeds. Eight stages of rice seeds variety MR 219 were tested for their callus induction response. By manipulating stages of seed the experiment was conducted to observe their effect on callus response. The stages were: immature/milky half mature mature fresh harvested or 1-6 months after harvest. The callus induction was on the MS basal medium with 30g/L sucrose 3.2g/L phytagel and 1 mg/L 2 4-D in combination with 10 mg/L NAA. Callus responses were obtained for each of the tested stages with a maximum at half mature stage 2.5g callus/plate. The used of seed for callus induction at five months-old and above are not recommended due to the reduction infresh weight of callus.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2008 Call Number |
| AGROVOC Term: | Callus |
| AGROVOC Term: | Rice |
| AGROVOC Term: | Seeds |
| AGROVOC Term: | Tissue culture |
| AGROVOC Term: | Weight |
| AGROVOC Term: | Tissue culture |
| AGROVOC Term: | Maturity |
| AGROVOC Term: | Plant embryos |
| AGROVOC Term: | Plant response |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12781 |
Actions (login required)
 |
View Item |
