Citation
Tan C. S., . and Gan K. S., . and Wee C. Y., . (2008) Construction of plant expression vector for biopharming of nipah virus surface glycoprotein. [Proceedings Paper]
Abstract
In the process of producing Nipah Virus NiV vaccine derived from plant a gene cassette of Nipah virus glycoprotein in plant expression vector was constructed. The Nipah Virus surface glycoprotein gene was obtained by RT-PCR using total RNA from virus culture lysate as a template. The 1.8 kb PCR amplified G gene was then cloned together with cMyc and 6xHis fusion tag at N-terminal into an intermediate vector to obtain CaMV 35Spromoter at 5 ;-end and Nos Poly-A terminator at 3 ;-end. After the verification by restriction enzymes and DNA sequencing the gene cassette was further sub-cloned into pCAMBIA2300 vector. The constructed plasmid was then transformed into Agrobacterium tumefaciens strain LBA4404 and was now ready for plant transformation
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Abstract
In the process of producing Nipah Virus NiV vaccine derived from plant a gene cassette of Nipah virus glycoprotein in plant expression vector was constructed. The Nipah Virus surface glycoprotein gene was obtained by RT-PCR using total RNA from virus culture lysate as a template. The 1.8 kb PCR amplified G gene was then cloned together with cMyc and 6xHis fusion tag at N-terminal into an intermediate vector to obtain CaMV 35Spromoter at 5 ;-end and Nos Poly-A terminator at 3 ;-end. After the verification by restriction enzymes and DNA sequencing the gene cassette was further sub-cloned into pCAMBIA2300 vector. The constructed plasmid was then transformed into Agrobacterium tumefaciens strain LBA4404 and was now ready for plant transformation
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2008 Call Number |
| AGROVOC Term: | Vectors |
| AGROVOC Term: | Viruses |
| AGROVOC Term: | Glycoproteins |
| AGROVOC Term: | Vaccines |
| AGROVOC Term: | PCR |
| AGROVOC Term: | RNA |
| AGROVOC Term: | Paramyxoviridae |
| AGROVOC Term: | Disease outbreak |
| AGROVOC Term: | Cloning |
| AGROVOC Term: | Agrobacterium tumefaciens |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12783 |
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