Citation
Hazniza A., . and Nor Azizun R., . and Sanimah S., . and Radzali M., . (2004) Preliminary study on growth performance of Micheua alba callus cultures. [Proceedings Paper]
Abstract
A preliminary study on growth performance ofMichelia alba or known as ;cempaka putih; callus cultures was studied. Callus of M.alba was obtained by inoculating the M.alba explant green petal of stage 5 on a basal Murashige and Skoog MS media supplemented with phytohormones antibrowning agent and different concentration of casein hydrolysate CH 25 50 100 and 150 mg/L w/v. The growth performance of of M.alba callus culture on two type of solidifying agents namely Gelrite and Kelcogel was then studied. The growth profile of the callus cultures was monitored for 30 days with 6 days interval between each observation. In each observation weight of callus culture was measured on dry weight basis. The growth of M.alba callus cultures in Gelrite was found highest at 30 days of observation in all the cultures that supplemented with 25 50 100 and 150 mg/L w/v casein hydrolysate. However the growth of callus cultures in Kelcogel was only highest in 50 and 150 mg/L w/v casein hydrolysate at 12 days of observation with 0.1060.02g and 0.097 O.017g dwt/culture respectively. Therefore in this study Gelrite is more preferable than Kelcogel prior to its high capability in propagating the callus cultures of M. alba based on dry weight basis per culture.
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Abstract
A preliminary study on growth performance ofMichelia alba or known as ;cempaka putih; callus cultures was studied. Callus of M.alba was obtained by inoculating the M.alba explant green petal of stage 5 on a basal Murashige and Skoog MS media supplemented with phytohormones antibrowning agent and different concentration of casein hydrolysate CH 25 50 100 and 150 mg/L w/v. The growth performance of of M.alba callus culture on two type of solidifying agents namely Gelrite and Kelcogel was then studied. The growth profile of the callus cultures was monitored for 30 days with 6 days interval between each observation. In each observation weight of callus culture was measured on dry weight basis. The growth of M.alba callus cultures in Gelrite was found highest at 30 days of observation in all the cultures that supplemented with 25 50 100 and 150 mg/L w/v casein hydrolysate. However the growth of callus cultures in Kelcogel was only highest in 50 and 150 mg/L w/v casein hydrolysate at 12 days of observation with 0.1060.02g and 0.097 O.017g dwt/culture respectively. Therefore in this study Gelrite is more preferable than Kelcogel prior to its high capability in propagating the callus cultures of M. alba based on dry weight basis per culture.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2004 Call Number |
| AGROVOC Term: | Michelia |
| AGROVOC Term: | Callus |
| AGROVOC Term: | Callus culture |
| AGROVOC Term: | Buds |
| AGROVOC Term: | Flower induction |
| AGROVOC Term: | Casein |
| AGROVOC Term: | Field experiments |
| AGROVOC Term: | Measurement |
| AGROVOC Term: | Weight |
| AGROVOC Term: | Growth |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12796 |
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