Citation
Lim L. S., . and Radzali Muse, . and Johari Ramli, . and Mohd Aspollah S., . (2004) Composition of essential oils extracted from the fresh fruits and leaves of Clausena excavata and their antibacterial activity. [Proceedings Paper]
Abstract
Essential oils were biochemically extracted with dichloromethane from the appropriate amount of fresh fruits and leaves of Clausena excavata by using simultaneous distillation extraction SDE technique. The oil yield obtained from fresh fruits was 0.092 while the oil yielded from fresh leaves was slightly lower than that of fruits only 0.064. In the GC-MS analysis only 20 biochemical components were identified in the volatile fruit oil while 22 biochemical components were identified in the volatile leaf oil. Terpenoids monoterpenes were the main group of biochemical components detected properly in the C. excavata fruit oil. The major biochemical components identified in the volatile fruit oil were -ocimene 42.06 and terpinolene 27.67 followed by y-terpinene 8.05 and linalool 5.99. Meanwhile terpinolene with remarkably high contain 42.35 also being the main component in C. excavata volatile leaf oil followed by some important amounts of sesquiterpenes such as farnesol 15.76 and germacrene-D 5.31. Those essential oils were aseptically subjected to antibacterial test using disc diffusion assay. Both of the volatile fruit and leaf oils of C. excavata showed slightly effects on all tested gram-positive bacteria and certain gram-negative bacteria. Among the tested bacteria strains Micrococcus luteus was considered effectively inhibited by fruit oil followed by Proteus vulgaris and Escherichia coli with inhibition zone diameters up to 10.00.0 mm including the disc diameter of 7 mm. Generally volatile fruit oil exhibited slightly better antibacterial activity compared to volatile leaf oil. They may potent as biostatic antibiotic for controlling the pathogenic bacteria growth and development.
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Abstract
Essential oils were biochemically extracted with dichloromethane from the appropriate amount of fresh fruits and leaves of Clausena excavata by using simultaneous distillation extraction SDE technique. The oil yield obtained from fresh fruits was 0.092 while the oil yielded from fresh leaves was slightly lower than that of fruits only 0.064. In the GC-MS analysis only 20 biochemical components were identified in the volatile fruit oil while 22 biochemical components were identified in the volatile leaf oil. Terpenoids monoterpenes were the main group of biochemical components detected properly in the C. excavata fruit oil. The major biochemical components identified in the volatile fruit oil were -ocimene 42.06 and terpinolene 27.67 followed by y-terpinene 8.05 and linalool 5.99. Meanwhile terpinolene with remarkably high contain 42.35 also being the main component in C. excavata volatile leaf oil followed by some important amounts of sesquiterpenes such as farnesol 15.76 and germacrene-D 5.31. Those essential oils were aseptically subjected to antibacterial test using disc diffusion assay. Both of the volatile fruit and leaf oils of C. excavata showed slightly effects on all tested gram-positive bacteria and certain gram-negative bacteria. Among the tested bacteria strains Micrococcus luteus was considered effectively inhibited by fruit oil followed by Proteus vulgaris and Escherichia coli with inhibition zone diameters up to 10.00.0 mm including the disc diameter of 7 mm. Generally volatile fruit oil exhibited slightly better antibacterial activity compared to volatile leaf oil. They may potent as biostatic antibiotic for controlling the pathogenic bacteria growth and development.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2004 Call Number |
| AGROVOC Term: | Clausena |
| AGROVOC Term: | Rutaceae |
| AGROVOC Term: | Fresh fruits |
| AGROVOC Term: | Leaves |
| AGROVOC Term: | Essential oils |
| AGROVOC Term: | Extraction |
| AGROVOC Term: | Extraction in chemical analysis |
| AGROVOC Term: | Distillation |
| AGROVOC Term: | Gas chromatography |
| AGROVOC Term: | Monoterpenes |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12809 |
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