Citation
Umi Salamah R., . and Abrizah O., . and Ravigadevi S., . (2004) Biochemical studies of lipid biosynthesis in oil palm Elaeis guineensis mesocarp. [Proceedings Paper]
Abstract
Fatty acid synthesis in plants occurs exclusively in the plastid. Although the enzymology of fatty acid synthesis in plants is well understood little is known about how carbon flux through the pathway is regulated. We have studied the role of different biochemical factors that control fatty acid composition in the oil palm mesocarp. Our observations suggest that fatty acid composition of palm oil was not controlled by a single enzyme. At least two different enzymes namely -ketoacyl formed per ACP synthase KAS II and palmitoyl ACP thioesterase control the levels of palmitate and oleate. Metabolic flux control analysis was applied to the oil palm previously mesocarp to examine distribution of control over carbon flux from acetate to fatty for acid biosynthesis and finally to complex lipid assembly via the Kennedy pathway. The rates of carbon incorporation from 14Cacetate to total lipid and acyl-CoAs pool were compared following specific enzyme inhibition to identify potential sites of regulation between fatty acid biosynthesis and the complex lipid assembly. By the use of top-down control analysis TDCA fatty acid biosynthesis has been shown to exert more control about 60 of control than complex lipid assembly under different experimental conditions. Nevertheless our results also emphasized that approx. 40 of the total control resides in the complex lipid assembly reaction.
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Abstract
Fatty acid synthesis in plants occurs exclusively in the plastid. Although the enzymology of fatty acid synthesis in plants is well understood little is known about how carbon flux through the pathway is regulated. We have studied the role of different biochemical factors that control fatty acid composition in the oil palm mesocarp. Our observations suggest that fatty acid composition of palm oil was not controlled by a single enzyme. At least two different enzymes namely -ketoacyl formed per ACP synthase KAS II and palmitoyl ACP thioesterase control the levels of palmitate and oleate. Metabolic flux control analysis was applied to the oil palm previously mesocarp to examine distribution of control over carbon flux from acetate to fatty for acid biosynthesis and finally to complex lipid assembly via the Kennedy pathway. The rates of carbon incorporation from 14Cacetate to total lipid and acyl-CoAs pool were compared following specific enzyme inhibition to identify potential sites of regulation between fatty acid biosynthesis and the complex lipid assembly. By the use of top-down control analysis TDCA fatty acid biosynthesis has been shown to exert more control about 60 of control than complex lipid assembly under different experimental conditions. Nevertheless our results also emphasized that approx. 40 of the total control resides in the complex lipid assembly reaction.
Additional Metadata
Item Type: | Proceedings Paper |
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Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2004 Call Number |
AGROVOC Term: | Elaeis guineensis |
AGROVOC Term: | Mesocarp |
AGROVOC Term: | Oil palm |
AGROVOC Term: | biochemical compounds |
AGROVOC Term: | Fatty acids |
AGROVOC Term: | Lipid content |
AGROVOC Term: | Iodine |
AGROVOC Term: | Biosynthesis |
AGROVOC Term: | Enzyme activity |
AGROVOC Term: | Biological control agents |
Geographical Term: | MALAYSIA |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 24 Apr 2025 05:15 |
URI: | http://webagris.upm.edu.my/id/eprint/12810 |
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