Callus induction on a basal medium of Murashige and Skoog supplemented with plant growth regulators by using different explants of Citrus grandis L. Osbeck

Rafidah S., . and Suryani Ahmad, . and Johari Ramli, . and Radzali M., . (2004) Callus induction on a basal medium of Murashige and Skoog supplemented with plant growth regulators by using different explants of Citrus grandis L. Osbeck. [Proceedings Paper]

This preliminary study was performed in order to induce callus on different concentrations of plant growth regulators supplied to a basal medium of Murashige and Skoog by using young leaf stem root and peel explant of Citrus grandis. According to the results obtained they showed that the basal medium of Murashige and Skoog MS 1962 containing 3.0g/L w/v of gelrite 30g/L w/v sucrose pH 5.7 supplemented with different concentrations of plant growth regulators such as Kinetin KN 2 4-dichlorophenoxy acetic acid 2 4-D and naphthalene acetic acid NAA were capable initiated callus derived from young leaf stem root and peel explants of Citrus grandis. Calluses were more succesfully induced and proliferated from young leaf and peel explants on the basal medium of Murashige and Skoog supplemented with KN 3 mg/L and 2 4-D 3 mg/L. A combination of KN 1 mg/L and NAA 2 mg/L are the best plant growth regulators for induction of callus from stem explant of Citrus grandis. Meanwhile a combination of KN l mg/L and 2 4 D 3 mg/L is the best plant growth regulators for induction of callus from young root explant of Citrus grandis. Most of calluses were friable compact texture and yellowish-white in colour for all young explants because they were actually meristematic tissues which were more suitable for the plant tissue culture systems.

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Abstract

This preliminary study was performed in order to induce callus on different concentrations of plant growth regulators supplied to a basal medium of Murashige and Skoog by using young leaf stem root and peel explant of Citrus grandis. According to the results obtained they showed that the basal medium of Murashige and Skoog MS 1962 containing 3.0g/L w/v of gelrite 30g/L w/v sucrose pH 5.7 supplemented with different concentrations of plant growth regulators such as Kinetin KN 2 4-dichlorophenoxy acetic acid 2 4-D and naphthalene acetic acid NAA were capable initiated callus derived from young leaf stem root and peel explants of Citrus grandis. Calluses were more succesfully induced and proliferated from young leaf and peel explants on the basal medium of Murashige and Skoog supplemented with KN 3 mg/L and 2 4-D 3 mg/L. A combination of KN 1 mg/L and NAA 2 mg/L are the best plant growth regulators for induction of callus from stem explant of Citrus grandis. Meanwhile a combination of KN l mg/L and 2 4 D 3 mg/L is the best plant growth regulators for induction of callus from young root explant of Citrus grandis. Most of calluses were friable compact texture and yellowish-white in colour for all young explants because they were actually meristematic tissues which were more suitable for the plant tissue culture systems.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2004 Call Number
AGROVOC Term:	Citrus grandis
AGROVOC Term:	Rutaceae
AGROVOC Term:	Callus culture
AGROVOC Term:	Plant growth regulators
AGROVOC Term:	Leaf extracts
AGROVOC Term:	Stems
AGROVOC Term:	Roots
AGROVOC Term:	Peel
AGROVOC Term:	Explants
AGROVOC Term:	Sterilizing
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/12816

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