Citation
Kodi I. K., . and Yohannes A. M., . and Abdul Ghani Y., . and Yazid M. M., . and Norihan M. S., . (2004) Morphological and histological assessment of callus induced from leaf explants of Sentang. [Proceedings Paper]
Abstract
Callus cultures were initiated from leaf explants of Sentang Azadirachta excelsa Jacob cultured on an induction medium: Murashige and Skoog;s medium supplemented with casein hydrolysate ascrobic acid three amino acids and auxin to cytokinin combinations. Leaf discs spontaneously developed callus and callusing frequency varied between 5-80 depending on the treatments applied. Two types of calli were observed: Callus type1 which was compact nodular pale yellow to creamy white and Callus Type 2 which was Semi-friable pale yellow to yellowish Azadiratwhite callus. Microscopy studies have also revealed morphologically different cellular characteristics of the two calli. Type1 calli were composed of isodiametric thin walled cells and randomly dispersed groups of smaller dividing cells i.e.meristem centers. Conversely Type 2 calli contained smaller sized cells appearing in clusters and each having a prominently larger nucleus.
Download File
Full text available from:
|
Abstract
Callus cultures were initiated from leaf explants of Sentang Azadirachta excelsa Jacob cultured on an induction medium: Murashige and Skoog;s medium supplemented with casein hydrolysate ascrobic acid three amino acids and auxin to cytokinin combinations. Leaf discs spontaneously developed callus and callusing frequency varied between 5-80 depending on the treatments applied. Two types of calli were observed: Callus type1 which was compact nodular pale yellow to creamy white and Callus Type 2 which was Semi-friable pale yellow to yellowish Azadiratwhite callus. Microscopy studies have also revealed morphologically different cellular characteristics of the two calli. Type1 calli were composed of isodiametric thin walled cells and randomly dispersed groups of smaller dividing cells i.e.meristem centers. Conversely Type 2 calli contained smaller sized cells appearing in clusters and each having a prominently larger nucleus.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2004 Call Number |
| AGROVOC Term: | Azadirachta |
| AGROVOC Term: | Leaves |
| AGROVOC Term: | Explants |
| AGROVOC Term: | Callus culture |
| AGROVOC Term: | Casein |
| AGROVOC Term: | Ascorbic acid |
| AGROVOC Term: | Amino acids |
| AGROVOC Term: | Auxins |
| AGROVOC Term: | Cytokinins |
| AGROVOC Term: | Plant histology |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12817 |
Actions (login required)
 |
View Item |
