Citation
Janna Ong Abdullah, . and Maziah M., . and Ahmad Parveez G. K. A., . and S. Kadzimin, . (2004) Evaluation of biological factors affecting gusA gene expression in Dendrobium Sonia protocorm-like-body. [Proceedings Paper]
Abstract
Seven biological factors affecting gusA gene expression in protocorm-like-body PLB of Dendrobium Sonia were tested. Three-week-old PLB ranging from 3 to 6 mm in size had significantly higher transient GUS activity and PLB of different genotypes exhibited different GUS expression rate. High transient gusA gene expressions were also observed in PLB sub-cultured onto fresh medium prior to transformation with 1.5g DNA. The gusA gene expression was maximal 2 days post-bombardment. Treatment of PLB with sorbitol or mannitol prior bombardment however had no significant effects on gusA gene activity.
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Abstract
Seven biological factors affecting gusA gene expression in protocorm-like-body PLB of Dendrobium Sonia were tested. Three-week-old PLB ranging from 3 to 6 mm in size had significantly higher transient GUS activity and PLB of different genotypes exhibited different GUS expression rate. High transient gusA gene expressions were also observed in PLB sub-cultured onto fresh medium prior to transformation with 1.5g DNA. The gusA gene expression was maximal 2 days post-bombardment. Treatment of PLB with sorbitol or mannitol prior bombardment however had no significant effects on gusA gene activity.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2004 Call Number |
| AGROVOC Term: | Dendrobium |
| AGROVOC Term: | Orchidaceae |
| AGROVOC Term: | Gene expression |
| AGROVOC Term: | DNA hybridization |
| AGROVOC Term: | Plasmids |
| AGROVOC Term: | Cell culture |
| AGROVOC Term: | Statistical analysis |
| AGROVOC Term: | Genetic transformation |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12824 |
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